Author: Jong N. Kim

Publisher:

Published: 2010

Total Pages:

ISBN-13:

Three different types of GS from P. ruminicola 23 were heterologously expressed in E. coli and characterized. Two different GS type III proteins (GSIII-1; ORFB01459 and GSIII-2; ORFB02034) showed GS biosynthetic activities, while no GSI biosynthetic activity was detected. We have defined the optimal conditions for enzymatic activity and these were found to be consistent with the parameters of the rumen habitat of P. ruminicola 23. Finally, we analyzed the effects on transcription and assayed key enzymatic activities on limiting (0.7 mM) and non-limiting (10 mM) concentrations of ammonia during growth in chemostat culture. These studies revealed an important role for GSIII-2 (ORFB02034) in P. ruminicola 23 when ammonia was excess. Changes in the global gene expression profile of P. ruminicola 23 in response to variations in the available nitrogen source (ammonia or peptides) were analyzed by microarray and related to changes in enzymatic activity and the proteome. In total, 110 genes (3.8% of the genome) were transcriptionally upregulated during growth on ammonia, while 120 genes (4.2 % of the genome) were transcriptionally upregulated during growth on peptides. P. ruminicola 23 grown on ammonia induced genes which are predicted to be involved in amino acid biosynthesis, molecular transport and several that affect the cell envelope. Growth on peptides induced genes whose products are involved in DNA metabolism, protein fate, protein synthesis and transcription. GS-GOGAT (GSIII-2: 22.5, GOGAT large subunit: 26.3, and small subunit: 22.4 fold) pathways was also upregulated when grown on ammonia. The greatest transcriptional up-regulation was observed for the ammonium transporter, amt (47.0 fold) and the nitrogen regulatory protein PII (46.5 fold) during growth with ammonia. In contrast, growth on peptides resulted in the up-regulation of more than 17 ribosomal proteins but no up-regulation of other nitrogen metabolism pathways. Our results provide a whole genome transcriptional overview of the responses by P. ruminicola 23 to two different nitrogen sources and are supported by proteomic and biochemical evidence. Collectively leading to an overall improvement in the understanding of the genetic responses and biochemical pathways used by P. ruminicola 23 to obtain and utilize different nitrogen sources. The response of P. ruminicola 23 to growth-limiting or non-limiting concentrations of ammonia were analyzed by microarray and related to changes in enzymatic activity. The results demonstrated dramatic changes in gene expression and enzymatic activity between the two conditions. In total, 166 genes (5.8% of the genome) were transcriptionally upregulated during growth on non-limiting concentration of ammonia, while 287 genes (10.0% of the genome) transcriptionally upregulated during growth on limiting concentration of ammonia. Specifically, growth in non-limiting concentrations of ammonia induced genes involved in amino acid biosynthesis, while ammonia limiting conditions caused the induction of genes involved in DNA metabolism, protein fate and the manipulation of the cell envelope. Links were observed between carbohydrate and nitrogen metabolism, such as between the reverse TCA cycle and glutamate biosynthesis during growth on non-limiting concentration of ammonia. Interestingly, P. ruminicola 23 grown on ammonia showed that ammonia assimilation pathways of NADPH-GDH and GS-GOGAT are major metabolic pathways on non-limiting concentration of ammonia. Especially, GSIII-2 and the ammonium transporter were significantly up-regulated on ammonia and these gene expression results reflected the enzyme assay results. Our studies provide a more detailed understanding of the genetic and enzymatic regulatory mechanisms of nitrogen metabolism undertaken by R. albus 8, a key fiber degrading ruminal Firmicute, and P. ruminicola 23, a predominant ruminal organism important in hemicellulose and protein metabolism.