Methods for Analysis of Carbohydrate Metabolism in Photosynthetic Organisms
Methods for Analysis of Carbohydrate Metabolism in Photosynthetic Organisms

Author: Horacio G Pontis

Publisher: Academic Press

Published: 2016-09-06

Total Pages: 252

ISBN-13: 0128034033

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Methods for Analysis of Carbohydrate Metabolism in Photosynthetic Organisms: Plants, Green Algae and Cyanobacteria examines both general and detailed aspects of carbohydrate metabolism in photosynthetic organisms, along with the four main oligosaccharides and each enzymatic reaction that gives birth to them. Chapters include information on how biological active protein is extracted for different cells, determination of enzymatic activity, separation of proteins by different available methods, and descriptions of analytical methods for the determination of various types of carbohydrates in photosynthetic organisms. The book contains useful protocols for researchers working on the determination of carbohydrate metabolism. The book provides foundational content as well as step-by-step guidance on how to design and conduct an experiment, including what other methodologies could be used if advanced instruments are not readily available. - Includes a variety of analytical methods and how to apply the methods using examples from specific case studies - Discusses technical information on how to characterize plant carbohydrates and sugar nucleosides - Contains easy-to-follow protocols with detailed explanations for self-guidance - Provides foundational content as well as step-by-step guidance on how to design and conduct an experiment

General carbohydrate method
General carbohydrate method

Author: Roy Whistler

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 634

ISBN-13: 0323144241

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Methods in Carbohydrate Chemistry, Volume VI: General Carbohydrate Methods contains a collection of selected methods from the entire field of carbohydrate chemistry. This volume is comprised of useful procedures in analytical and preparative carbohydrate chemistry. It is organized into 10 sections. The first section deals with methods for separation and analysis, which discusses chromatography and chemical, physical, and biochemical methods. Section II covers the preparation of mono- and polysaccharides and their derivatives. Section III describes a variety of oxidation methods. The fourth section is about procedures for the analysis of acyclic sugars. Sections V and VI present the etherification and esterification of carbohydrates. Nucleotides, nucleosides, and glycoside procedures are described in Sections VII and VIII. The ninth section focuses on radioactively labeled sugars. The final chapter provides a variety of physical methods such as mass spectrometry, nuclear magnetic resonance spectroscopy, and determination of molecular weights by osmometry. Chemists and biochemists will find this book very useful.

General Methods
General Methods

Author: Roy Whistler

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 373

ISBN-13: 032315364X

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Methods in Carbohydrate Chemistry, Volume VIII: General Methods describes the principles and mode of operation of general methods for the analysis and structural characterization of carbohydrates. This book is organized into two sections encompassing 50 chapters. The first section highlights the methods on automated chromatographic techniques; enzymic and other methods for structural analysis of polysaccharides. This section also explores the application of 13C NMR spectroscopy to carbohydrate chemistry. The second section describes the synthesis of deoxy and branched-chain sugars, 1,2-transglycosides, de-N- and de-O-sulfation, and de-N-acetylation of polysaccharides. This book is an invaluable source for organic and analytical chemists, as well as for carbohydrate scientists and researchers.

Recognition of Carbohydrates in Biological Systems, Part A: General Procedures
Recognition of Carbohydrates in Biological Systems, Part A: General Procedures

Author: Y. C. Lee

Publisher: Academic Press

Published: 2003-08-26

Total Pages: 625

ISBN-13: 9780121822651

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Recognition of carbohydrates in biological systems has been gaining more and more attention in recent years. Although methodology for studying recognition has been developing, there is no volume that covers the wide area of methodology of carbohydrate recognition. This volume, Recognition of Carbohydrates in Biological Systems, Part A: General Procedures, and its companion, Volume 363, present state-of-the-art methodologies, as well as the most recent biological observations in this area. Covers the isolation/synthesis of substances used in studying interactions involving carbohydrates Discussed the methodology for measuring such interactions Biological roles for such interactions are also covered

Glycoscience
Glycoscience

Author: Bertram O. Fraser-Reid

Publisher: Springer Science & Business Media

Published: 2008-04-14

Total Pages: 2847

ISBN-13: 3540361545

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As a reflection of the quantum leap that has been made in the study of glycostructures, the first edition of this book has been completely revised and updated. The editors give up-to-date information on glycostructures, their chemistry and chemical biology in the form of a completely comprehensive survey. Glycostructures play highly diverse and crucial roles in a myriad of organisms and important systems in biology, physiology, medicine, bioengineering and technology. Only in recent years have the tools been developed to partly understand the highly complex functions and the chemistry behind them. While many facts remain undiscovered, this MRW has been contributed to by a large number of the world’s leading researchers in the field.

The Art of Carbohydrate Analysis
The Art of Carbohydrate Analysis

Author: Gerrit J. Gerwig

Publisher: Springer Nature

Published: 2021-10-23

Total Pages: 362

ISBN-13: 303077791X

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The growing importance of glycobiology and carbohydrate chemistry in modern biotechnology and the pharmaceutical industry makes accurate carbohydrate analysis indispensable. This book provides the principles and protocols of various fundamental carbohydrate analysis methods. Choice of method is entirely dependent upon the type of material being investigated (biological samples, food products, etc.), and the level of structural detail required, i.e. sugar content, compositional analysis, linkages between the sugar components, or the total chemical structure of a given molecule. Full structural characterization of carbohydrate chains requires significant time, resources, and skill in several methods of analysis; no single technique can address all glycan analysis needs. This book summarizes several existing analytical techniques (both chemical and physical) in an introductory volume designed for the non-expert researcher or novice scientist. While background in carbohydrate chemistry is assumed, all information necessary to understanding the described techniques is addressed in the text.

Carbohydrate Analysis by Modern Liquid Phase Separation Techniques
Carbohydrate Analysis by Modern Liquid Phase Separation Techniques

Author: Ziad El Rassi

Publisher: Elsevier

Published: 2021-08-15

Total Pages: 956

ISBN-13: 0128245123

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Carbohydrate Analysis by Modern Liquid Phase Separation Techniques, Second Edition, presents readers with the various principles of modern liquid phase separation techniques and their contributions to the analysis of complex carbohydrates and glycoconjugates. In a selection of all-new chapters, this fully updated volume covers each technique in detail. The book aims to help analysts solve any of the many practical problems they may face in tackling the analysis of carbohydrates. In addition, it addresses current difficulties that must be resolved in carbohydrate research, thus inspiring further important technological developments to meet these challenges. This is an essential resource for anyone seeking a broad view of the science of carbohydrates and separation techniques. - Covers the basic principles of modern liquid phase separation techniques, along with their applications - Compiles up-to-date information on the field of carbohydrate analysis, along with updates on separation science - Focuses on problems currently faced in carbohydrate analysis and the solutions necessary for further progress