Protocol for Somatic Embryogenesis in Woody Plants

Protocol for Somatic Embryogenesis in Woody Plants

Author: Shri Mohan Jain

Publisher: Springer Science & Business Media

Published: 2005-05-23

Total Pages: 604

ISBN-13: 9781402029844

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World population is increasing at an alarming rate and this has resulted in increasing tremendously the demand for tree products such as wood for construction materials, fuel and paper, fruits, oils and medicines etc. This has put immense pressure on the world’s supplies of trees and raw material to industry and will continue to do so as long as human population continues to grow. Also, the quality of human diet, especially nutritional components, is adversely affected due to limited genetic improvement of most of fruit trees. Thus there is an immediate need to increase productivity of trees. Improvement has been made through conventional breeding methods, however, conventional breeding is very slow due to long life cycle of trees. A basic strategy in tree improvement is to capture genetic gain through clonal propagation. Clonal propagation via organogenesis is being used for the production of selected elite individual trees. However, the methods are labour intensive, costly, and produce low volumes. Genetic gain can now be captured through somatic embryogenesis. Formation of embryos from somatic cells by a process resembling zygotic embryogenesis is one of the most important features of plants. In 1958, Reinert in Germany and Steward in USA independently reported somatic embryogenesis in carrot cultures. Since then, tremendous progress in somatic embryogenesis of woody and non-woody plants has taken place. It offers a potentially large-scale propagation system for superior clones.


Micropropagation of Woody Plants

Micropropagation of Woody Plants

Author: M.R. Ahuja

Publisher: Springer Science & Business Media

Published: 1992-11-30

Total Pages: 540

ISBN-13: 9780792318071

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This volume covers recent advances in the vegetative propagation of woody plants by tissue culture. A wide range of topics relevant to micropropagation of woody plants are discussed by renowned international scientists. These include cellular contro of morphogenesis, light regimes in tissue culture, maturation and rejuvenation, synthetic seed, genetics of micropropagated plants, haploid embryogenesis, protoplast culture, and acclimatization of ex vitro woody plants. In addition to micropropagation of selected woody plants, both gymnosperms and angiosperms, this volume also includes in vitro genetic selection, strategic planning for application of biotechnology for genetics and breeding, and clonal options for woody plant improvement. A balanced view of both perspectives and limitations of woody plant micropropagation is presented.


Somatic Embryogenesis in Woody Plants

Somatic Embryogenesis in Woody Plants

Author: S.M. Jain

Publisher: Springer Science & Business Media

Published: 2013-11-11

Total Pages: 743

ISBN-13: 940173030X

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The quality of human life has been maintained and enhanced for generations by the use of trees and their products. In recent years, ever rising human population growth has put a tremendous pressure on trees and tree products; growing awareness of the potential of previously unexploited tree resources; and environ mental pollution have both accelerated the development of new technologies for tree propagation, breeding and improvement. Biotechnology of trees may be the answer to solve the problems which can not be solved by conventional breeding methods. The combination of biotechnology and conventional methods such as plant propagation and breeding may be a novel approach to improving and multiplying a large number of the trees and woody plants. So far, plant tissue culture technology has largely been exploited by commercial companies in propagation of ornamentals, especially foliage house plants. Gene rally, tissue culture of woody plants has been recalcitrant. However, limited success has been achieved in tissue culture of angiosperm and gymnosperm woody plants. A number of recent reports on somatic embryogenesis in woody plants such as Norway spruce (Picea abies), Loblolly pine (Pinus taedb), Sandalwood (Santalum album), Citrus, mango (Mangifera indica), etc. , offer a ray of hope of: a) inexpensive clonal propagation for large-scale production of plants or "emblings" or somatic seedlings; b) protoplast work; c) cryopreservation; d) genetic transformation; and e) synthetic or artificial or manufactured seed production.


Somatic Embryogenesis in Woody Plants

Somatic Embryogenesis in Woody Plants

Author: S.M. Jain

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 338

ISBN-13: 9401147744

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The quality of human life has been maintained and enhanced for generations by the use of trees and their products. In recent years, ever rising human population growth has put a tremendous pressure on trees and tree products; growing awareness of the potential of previously unexploited tree resources; and environmental pollution have both accelerated the development of new technologies for tree propagation, breeding and improvement. Biotechnology of trees may be the answer to solve the problems which can not be solved by conventional breeding methods. The combination of biotechnology and conventional methods such as plant propagation and breeding could become a novel approach to improving and multiplying a large number of the trees and woody plants. So far, plant tissue culture technology has largely been exploited by commercial companies in propagation of ornamentals, especially foliage house plants. Generally, tissue culture of woody plants has been recalcitrant. However, limited success has been achieved in tissue culture of angiosperm and gymnosperm woody plants. A number of recent reports on somatic embryogenesis in woody plants such as Norway spruce (Picea abies), Loblolly pine (Pinus taeda), Sandalwood (Santalum album), Citrus and mango (Mangifera indica), offer a ray of hope for inexpensive clonal propagation for large-scale production of plants or 'emblings' or somatic seedlings; protoplast work; cryopreservation; genetic transformation; and synthetic or artificial or manufactured seed production.


Somatic Embryogenesis in Woody Plants

Somatic Embryogenesis in Woody Plants

Author: S. Mohan Jain

Publisher: Springer

Published: 1995-05-31

Total Pages: 513

ISBN-13: 9780792330707

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The quality of human life has been maintained and enhanced for generations by the use of trees and their products. In recent years, ever rising human population growth has put tremendous pressure on trees and tree products; growing awareness of the potential of previously unexploited tree resources and environmental pollution have both accelerated development of new technologies for tree propagation, breeding and improvement. Biotechnology of trees may be the answer to solve the problems which cannot be solved by conventional breeding methods. The combination of biotechnology and conventional methods such as plant propagation and breeding may be a novel approach to improving and multiplying in large number the trees and woody plants. So far, plant tissue culture technology has largely been exploited in the propagation of ornamental plants, especially foliage house plants, by com mercial companies. Generally, tissue culture of woody plants has been recal citrant. However, limited success has been achieved in tissue culture of angiosperm and gymnosperm woody plants. A number of recent reports on somatic embryogenesis in woody plants such as Norway spruce (Picea abies), Loblolly pine (Pinus taeda), Sandalwood (Santalurn album), Citrus, Mango (Mangifera indica), etc. , offer a ray of hope of: a) inexpensive clonal propa gation for large-scale production of plants or "emblings" or "somatic embryo plants", b) protoplast work, c) cryopreservation, d) genetic transformation, and e) artificial or manufactured seed production.