The FISH Handbook for Biological Wastewater Treatment provides all the required information for the user to be able to identify and quantify important microorganisms in activated sludge and biofilms by using fluorescence in situ hybridization (FISH) and epifluorescence microscopy. It has for some years been clear that most microorganisms in biological wastewater systems cannot be reliably identified and quantified by conventional microscopy or by traditional culture-dependent methods such as plate counts. Therefore, molecular biological methods are vital and must be introduced instead of, or in addition to, conventional methods. At present, FISH is the most widely used and best tested of these methods. This handbook presents all relevant information from the literature and, based on the extensive experience of the authors, advice and recommendations are given for reliable FISH identification and quantification. The overall purpose of the book is to help scientists, consultants, students, and plant operators to get an overview of important microorganisms in biological wastewater treatment and to explain how FISH can be used for detecting and quantifying these microbes. A proper and reliable identification of dominant microorganisms is of great importance for research and new developments in the wastewater treatment industry, and it is important for optimization and troubleshooting of operational problems in present wastewater treatment plants. The book encompasses an overview of dominant microorganisms present in the wastewater treatment systems, which oligonucleotide probes (gene probes) to select for detection of these microbes by FISH, how to perform FISH (detailed protocols), how to quantify the microbes, and how to solve common problems of FISH. The book addresses several functional groups: nitrifiers, denitrifiers, polyphosphate-accumulating organisms, glycogen-accumulating organisms, bacteria involved in hydrolysis and fermentation, filamentous bacteria from bulking sludge, and scum-forming bacteria. A comprehensive collection of FISH-images showing dominant representatives of these groups helps readers to use FISH in the context of wastewater treatment.
This book presents recent developments in advanced biological treatment technologies that are attracting increasing attention or that have a high potential for large-scale application in the near future. It also explores the fundamental principles as well as the applicability of the engineered bioreactors in detail. It describes two of the emerging technologies: membrane bioreactors (MBR) and moving bed biofilm reactors (MBBR), both of which are finding increasing application worldwide thanks to their compactness and high efficiency. It also includes a chapter dedicated to aerobic granular sludge (AGS) technology, and discusses the main features and applications of this promising process, which can simultaneously remove organic matter, nitrogen and phosphorus and is considered a breakthrough in biological wastewater treatment. Given the importance of removing nitrogen compounds from wastewater, the latest advances in this area, including new processes for nitrogen removal (e.g. Anammox), are also reviewed. Developments in molecular biology techniques over the last twenty years provide insights into the complex microbial diversity found in biological treatment systems. The final chapter discusses these techniques in detail and presents the state-of-the-art in this field and the opportunities these techniques offer to improve process performance.
The first edition of this book was published in 2008 and it went on to become IWA Publishing’s bestseller. Clearly there was a need for it because over the twenty years prior to 2008, the knowledge and understanding of wastewater treatment had advanced extensively and moved away from empirically-based approaches to a fundamental first-principles approach based on chemistry, microbiology, physical and bioprocess engineering, mathematics and modelling. However the quantity, complexity and diversity of these new developments was overwhelming for young water professionals, particularly in developing countries without readily available access to advanced-level tertiary education courses in wastewater treatment. For a whole new generation of young scientists and engineers entering the wastewater treatment profession, this book assembled and integrated the postgraduate course material of a dozen or so professors from research groups around the world who have made significant contributions to the advances in wastewater treatment. This material had matured to the degree that it had been codified into mathematical models for simulation with computers. The first edition of the book offered, that upon completion of an in-depth study of its contents, the modern approach of modelling and simulation in wastewater treatment plant design and operation could be embraced with deeper insight, advanced knowledge and greater confidence, be it activated sludge, biological nitrogen and phosphorus removal, secondary settling tanks, or biofilm systems. However, the advances and developments in wastewater treatment have accelerated over the past 12 years since publication of the first edition. While all the chapters of the first edition have been updated to accommodate these advances and developments, some, such as granular sludge, membrane bioreactors, sulphur conversion-based bioprocesses and biofilm reactors which were new in 2008, have matured into new industry approaches and are also now included in this second edition. The target readership of this second edition remains the young water professionals, who will still be active in the field of protecting our precious water resources long after the aging professors who are leading some of these advances have retired. The authors, all still active in the field, are aware that cleaning dirty water has become more complex but that it is even more urgent now than 12 years ago, and offer this second edition to help the young water professionals engage with the scientific and bioprocess engineering principles of wastewater treatment science and technology with deeper insight, advanced knowledge and greater confidence built on stronger competence.
Over the past twenty years, the knowledge and understanding of wastewater treatment has advanced extensively and moved away from empirically based approaches to a fundamentally-based first principles approach embracing chemistry, microbiology, and physical and bioprocess engineering, often involving experimental laboratory work and techniques. Many of these experimental methods and techniques have matured to the degree that they have been accepted as reliable tools in wastewater treatment research and practice. For sector professionals, especially a new generation of young scientists and engineers entering the wastewater treatment profession, the quantity, complexity and diversity of these new developments can be overwhelming, particularly in developing countries where access to advanced level laboratory courses in wastewater treatment is not readily available. In addition, information on innovative experimental methods is scattered across scientific literature and only partially available in the form of textbooks or guidelines. This book seeks to address these deficiencies. It assembles and integrates the innovative experimental methods developed by research groups and practitioners around the world. Experimental Methods in Wastewater Treatment forms part of the internet-based curriculum in wastewater treatment at UNESCO-IHE and, as such, may also be used together with video records of experimental methods performed and narrated by the authors including guidelines on what to do and what not to do. The book is written for undergraduate and postgraduate students, researchers, laboratory staff, plant operators, consultants, and other sector professionals.
Wastewater Treatment: Molecular Tools, Techniques, and Applications provides an insight about the application of different tools and technology for exploring microbial structure-function relationships that involved in WWTPs. From the present day consequence of alarming usable water crysis throughout the globe, an immediate action on water cycle is necessary. Along with other options the waste water recycling is one major opportunity to combat the future scarcity. The book aims to provide a comprehensive view of advanced emerging technologies for wastewater treatment, heavy metal removal, pesticide degradation, dye removal, waste management, microbial transformation of environmental contaminants, etc. It also describes different application of Omic tools in Waste water treatment plants (WWTPs),describes the role of microorganisms in WWTPs, points out the reuse of treated wastewater through emerging technologies, also includes the recovery of resources from wastewater and emphasizes on cutting edge molecular tools for WWTPs. We hope the content of the book will be very much usefull for the community who are directly associated in wastewater management research, people who are associated with environmental awarness programme and the students of UG and PG courses. Features: This book highlights the importance of molecular genomics, molecular biology techniques to sort out the problems faced by industrialist who operates wastewater treatment plant with the ever-increasing number of environmental pollutants. Describes application of different Omic tools in Wastewater treatment plants (WWTPs) Describes the role of microorganisms in WWTPs Points out the reuse of treated wastewater through emerging technologies. Includes the recovery of resources from wastewater Emphasizes on cutting edge molecular tools This book targets engineers, scientists and managers who require an excellent introduction and basic knowledge to the principles of molecular biology or molecular genomics in the area of wastewater treatment. Different professionals working or interested in the Environmental Microbiology or Bioremediation or Environmental Genomics field. Students on Environmental Biotechnology/Microbiology.
The scope of this comprehensive new edition of Handbook of Biological Wastewater Treatment ranges from the design of the activated sludge system, final settlers, auxiliary units (sludge thickeners and digesters) to pre-treatment units such as primary settlers and UASB reactors. The core of the book deals with the optimized design of biological and chemical nutrient removal. The book presents the state-of-the-art theory concerning the various aspects of the activated sludge system and develops procedures for optimized cost-based design and operation. It offers a truly integrated cost-based design method that can be easily implemented in spreadsheets and adapted to the particular needs of the user. Handbook of Biological Wastewater Treatment: Second Edition incorporates valuable new material that improves the instructive qualities of the first edition. The book has a new structure that makes the material more readily understandable and the numerous additional examples clarify the text. On the website www.wastewaterhandbook.com three free excel design spreadsheets for different configurations (secondary treatment with and without primary settling and nitrogen removal) can be downloaded to get the reader started with their own design projects. New sections have been added throughout: to explain the difference between true and apparent yield while the section on the F/M ratio, and especially the reasons not to use it, has been expanded; to demonstrate the effect of the oxygen recycle to the anoxic zones on both the denitrification capacity and the concept of available nitrate is explained in more detail. the latest developments on the causes and solution to sludge bulking and scum formation to show the rapid developments of innovative nitrogen removal and sludge separation problems the anaerobic pre-treatment section is completely rewritten based on the experiences obtained from an extensive review of large full-scale UASB based sewage treatment plants a new section on industrial anaerobic wastewater treatment three new appendices have been added. These deal with the calibration of the denitrification model, empirical design guidelines for final settler design (STORA/STOWA and ATV) and with the potential for development of denitrification in the final settler. A new chapter on moving bed biofilm reactors Handbook of Biological Wastewater Treatment: Second Edition is written for post graduate students and engineers in consulting firms and environmental protection agencies. It is an invaluable resource for everybody working in the field of wastewater treatment. Lecturer support material is available when adopted for university courses. This includes course material for the first 7 modules in the form of PDF printouts and an exercise file with questions and answers and a symbol list. Authors: Prof. dr. ir. A.C. van Haandel, Federal University of Campina Grande - Brazil and Ir. J.G.M. van der Lubbe, Biothane Systems International - Veolia, The Netherlands
The enhanced biological removal of phosphorus (EBPR) is a popular process due to high removal efficiency, low operational costs, and the possibility of phosphorus recovery. Nevertheless, the stability of the EBPR depends on different factors such as: temperature, pH, and the presence of toxic compounds. While extensive studies have researched the effects of temperature and pH on EBPR systems, little is known about the effects of different toxic compounds on EBPR. For example, sulphide has shown to inhibit different microbial activities in the WWTP, but the knowledge about its effects on EBPR is limited. Whereas the sulphide generated in the sewage can cause a shock effect on EBPR, the continuously exposure to sulphide potentially generated in WWTP can cause the acclimatization and adaptation of the biomass. This research suggests that sulphate reducing bacteria can proliferate in WWTP, as they are reversibly inhibited by the recirculation of sludge through anaerobic-anoxic-oxic conditions. The research enhances the understanding of the effect of sulphide on the anaerobic-oxic metabolism of PAO. It suggests that the filamentous bacteria Thiothrix caldifontis could play an important role in the biological removal of phosphorus. It questions the ability of PAO to generate energy from nitrate respiration and its use for the anoxic phosphorus uptake. Thus, the results obtained in this research can be used to understand the stability of the EBPR process under anaerobic-anoxic-oxic conditions, especially when exposed to the presence of sulphide.
Propionic acid is an important intermediate produced during anaerobic degradation of biowaste and a precursor of a large amount of methane. Its accumulation during biomethanation is however a common problem resulting in stagnation phases in biogas production. During this study, the deeper insight into the process of propionic acid degradation was intended by using modern analytical chemistry, standard microbiological approach and molecular biology for describing and explaining the problem.
Handbook on Organic Waste for Biological Treatment, Liquid Manure into a Solid, Tomato Waste Water Treatment, Oxalic Acid from Jute Stick, Cotton Processing Waste, Fish Waste, Agro-Industrial Wastes, Bioconversion of Pretreated Wheat Straw and Sunflower Stalks to Ethanol, Agricultural Waste Treatment, Waste of Dehydrated Onion, Beef-Cattle Manure Slurry, Meat Meal and Algae for Calves, Wastes from Large Piggeries, Pig Waste, Oxytetracycline, Methane from Cattle Waste (Also Known as The Complete Book on Biological Waste Treatment and their Utilization) Biological Treatment is the recycling of humus, nutrients and/or energy from biological waste by means of aerobic (composting) or anaerobic (digesting) processing. Biological treatment is an important and integral part of any wastewater treatment plant that treats wastewater from either municipality or industry having soluble organic impurities or a mix of the two types of wastewater sources. Biological wastewater treatment is an important and integral step of wastewater treatment system and it treats wastewater coming from either residential buildings or industries etc. It is often called as Secondary Treatment process which is used to remove any contaminants that left over after primary treatment. Organic waste is material that is biodegradable and comes from either a plant or animal. Organic waste is usually broken down by other organisms over time and may also be referred to as wet waste. Most of the time, it's made up of vegetable and fruit debris, paper, bones and human waste which quickly disintegrate. Wastewater treatment is a process used to convert wastewater, which is water no longer needed or suitable for its most recent use, into an effluent that can be either returned to the water cycle with minimal environmental issues or reused. Expenditure on water and wastewater infrastructure in India is set to increase by 83% over the next five years, hitting an annual run rate of $16 billion by 2020. The utility market is set to top $14 billion within five years, while annual spending in the industrial sector will approach $2 billion. Spending on water supply will grow from $5.56 billion to $9.4 billion over the next five years. It will be a standard reference book for professionals, entrepreneurs, those studying and researching in this important area. TAGS Biological Treatment, Organic-Waste Treatment, Biological Treatment of Organic Waste, Biological Wastewater Treatment, Biological Treatment Plant or Organic Waste, Organic Solid Waste Biological Treatment, Biological Treatment Plant, Microorganisms in Organic Waste Disposal, Biological Treatment of Waste, Process for Biological Treatment of Organic Waste, Biological Treatment Process, Organic Waste Treatment, Organic Waste Recycling, Organic Waste Forms and Treatment Strategies, Biological Waste Treatment and Utilization, Transformation of Liquid Manure into Solid, Tomato Waste Water Treatment, Treatment of Wastewater from Peeled Tomato, Tomato Cleaning and Water Recycle, Preparation of Oxalic Acid from Jute Stick, Oxalic Acid Manufacture, Oxalic Acid from Jute Stick, Digestion of Cotton Processing Waste, Properties of Sorghum Stalk, Physical and Mechanical Properties of Sorghum Stalk, Biological Fermentation of Fish Waste, Fermentation of Fish Waste, Fermented Fish Waste, Fish Waste in Fermentation, Agro-industrial Wastes, Agro-industrial wastes utilization, Recycling of Agro-Industrial Wastes, Modelling of Agricultural Waste Treatments, Utilization of Waste of Dehydrated Onion, Utilization of Waste Products of Dehydrated Onion Industry, Palm Oil Mill Effluent Disposal on Land, Palm Oil Mill Effluent (POME), Palm Oil Mill Effluent (POME) Treatment, Waste Management in Palm Oil Mill, Management and Treatment of Wastes from Large Piggeries, treatment of wastes from piggeries, Treatment of Piggery Wastes, Management of Wastes from Pig, Piggery Waste Management, Tower Digestion of Pig Waste, Nutritive Value of Poultry Waste, Digestion of Rabbit and Pig Waste, Chemical Composition of Palm Oil Mill Effluent, Humic Substances from Composed Barks, Humic Substances from Decomposing Bark, Particle Size and Tomato Waste Digestion, Humic Acids on Hydrolysis of Potato Protein, Effects of Composts on Wheat Yields, Production of Oxytetracycline, Oxytetracycline Production, Production of oxytetracycline from agricultural wastes, Use of Manure in Fish Farming, Bacteria in Swine Waste, Poultry Waste Water as Broiler Feeds, Utilization of Indian Wastes in Livestock Feeds, Methane from Cattle Waste, Methane Production from Cattle Waste, Treatment of Milking Parlour Wastewater, Pig Liquid Manure, UASB Treatment of Wastes, Digestion of Poultry Litter, Beef-Cattle Manure Slurries, New small scale ideas for Biological Treatment, Business Ideas for Biological Treatment, How to start a Biological Treatment Plant, Start Your Own Biological Treatment Business, Biological Treatment Business Plan, Business plan for Organic Waste for Biological Treatment, Small Scale Industries in India, Organic Waste for Biological Treatment Based Small Business Ideas in India, Small Scale Industry You Can Start on Your Own, Business plan for small scale industries, Profitable Small Scale Manufacturing, How to Start a Small Business in India, Free Manufacturing Business Plans, Small and Medium Scale Manufacturing, Profitable Small Business Industries Ideas, Business ideas for Startup, Detailed Project Report on Biological Treatment, Project Report on Biological Treatment, Pre-Investment Feasibility Study on Oxytetracycline Production, Techno-Economic feasibility study on Oxytetracycline Production, Feasibility report on Piggery Waste Management, Free Project Profile on Piggery Waste Management, Project profile on Organic Waste for Biological Treatment, Download free project profile on Oxytetracycline Production
Activated sludge is the most widely used biological wastewater treatment process globally to date, although its high energy demand makes it a major contributor of greenhouse gas emissions. Over recent decades it has been constantly modified and retrofitted to treat ever higher loads or improve effluent standards which have often resulted in even greater carbon emissions. Conventional activated sludge treatment is at a crossroads where new sustainable solutions are required if we are to protect the quality of our rivers and meet net-zero carbon targets.The book details current operation and design with special emphasis on the biological aspects of the process. From the microbial kinetics to the fascinating process of floc formation and development, the book explores the development of our understanding of the process looking at new sustainable designs, including biological nutrient removal and new aeration systems. Sludge separation problems and control options are explained, with a trouble-shooting guide to non-bulking problems. Environmental issues including noise, odor, aerosols, micro-plastics and nanoparticles are all reviewed, as is pathogen removal and the problem of antibiotic resistant genes and bacteria. The development of membrane bioreactors has increased process reliability and effluent quality, while integrated fixed-film activated sludge processes are more efficient and compact. The book concludes by exploring how activated sludge can become more sustainable, for example, by carbon harvesting and byproduct recovery.This interdisciplinary book is essential reading for both engineers and scientists whether training at university or practitioners and consultants in the wastewater industry.Related Link(s)
