Field Guide to Microscopy

Field Guide to Microscopy

Author: Tomasz S. Tkaczyk

Publisher: SPIE-International Society for Optical Engineering

Published: 2010

Total Pages: 162

ISBN-13:

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This guide provides extensive coverage of microscopic imaging principles. After reviewing the main principles of image formation, diffraction, interference, and polarization used in microscopy, this guide describes the most widely applied microscope configurations and applications. It also covers major system components, including light sources, illumination layouts, microscope optics, and image detection electronics. This guide also provides a comprehensive overview of microscopy techniques, including bright field and dark field imaging, contrast enhancement methods (such as phase and amplitude contrast), DIC, polarization, and fluorescence microscopy. In addition, it describes scanning techniques (such as confocal and multiphoton imaging points); new trends in super-resolution methods (such as 4Pi microscopy, STED, STORM, and structured illumination); and array microscopy, CARS, and SPIM.


A Beginners' Guide to Scanning Electron Microscopy

A Beginners' Guide to Scanning Electron Microscopy

Author: Anwar Ul-Hamid

Publisher: Springer

Published: 2018-10-26

Total Pages: 422

ISBN-13: 3319984829

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This book was developed with the goal of providing an easily understood text for those users of the scanning electron microscope (SEM) who have little or no background in the area. The SEM is routinely used to study the surface structure and chemistry of a wide range of biological and synthetic materials at the micrometer to nanometer scale. Ease-of-use, typically facile sample preparation, and straightforward image interpretation, combined with high resolution, high depth of field, and the ability to undertake microchemical and crystallographic analysis, has made scanning electron microscopy one of the most powerful and versatile techniques for characterization today. Indeed, the SEM is a vital tool for the characterization of nanostructured materials and the development of nanotechnology. However, its wide use by professionals with diverse technical backgrounds—including life science, materials science, engineering, forensics, mineralogy, etc., and in various sectors of government, industry, and academia—emphasizes the need for an introductory text providing the basics of effective SEM imaging.A Beginners’ Guide to Scanning Electron Microscopy explains instrumentation, operation, image interpretation and sample preparation in a wide ranging yet succinct and practical text, treating the essential theory of specimen-beam interaction and image formation in a manner that can be effortlessly comprehended by the novice SEM user. This book provides a concise and accessible introduction to the essentials of SEM includes a large number of illustrations specifically chosen to aid readers' understanding of key concepts highlights recent advances in instrumentation, imaging and sample preparation techniques offers examples drawn from a variety of applications that appeal to professionals from diverse backgrounds.


A Field Guide to Bacteria

A Field Guide to Bacteria

Author: Betsey Dexter Dyer

Publisher: Comstock Publishing Associates

Published: 2003

Total Pages: 400

ISBN-13: 9780801488542

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Written for curious souls of all ages, this title opens readers eyes--and noses and ears--to this hidden world. Useful illustrations accompany Dyer's lively text.


The Microscope and How to Use It

The Microscope and How to Use It

Author: Dr. Georg Stehli

Publisher: Courier Corporation

Published: 2012-03-13

Total Pages: 162

ISBN-13: 048613721X

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In nontechnical language and with 199 photographs and drawings, the author clearly explains how a microscope works and what kind to use; preparation and examination of specimens, and much more.


Light and Video Microscopy

Light and Video Microscopy

Author: Randy O. Wayne

Publisher: Academic Press

Published: 2013-12-16

Total Pages: 373

ISBN-13: 0124115365

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The purpose of this book is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy will prepare the reader for the accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells, which influence interpretation. - Brings together mathematics, physics, and biology to provide a broad and deep understanding of the light microscope - Clearly develops all ideas from historical and logical foundations - Laboratory exercises included to assist the reader with practical applications - Microscope discussions include: bright field microscope, dark field microscope, oblique illumination, phase-contrast microscope, photomicrography, fluorescence microscope, polarization microscope, interference microscope, differential interference microscope, and modulation contrast microscope


Guide to Microlife

Guide to Microlife

Author: Kenneth G. Rainis

Publisher:

Published: 1996-01-01

Total Pages: 287

ISBN-13: 9780531112663

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Serves as a guide to be used for the identification of microorganisms and provides information about microlife forms and how they affect other life forms, including human.


Insect Microscopy

Insect Microscopy

Author: Andrew Chick

Publisher: The Crowood Press

Published: 2016-08-31

Total Pages: 213

ISBN-13: 1785002023

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Insects, and their close relatives, the arachnids, centipedes, millipedes and woodlice, make ideal material for study by the recreational microscopist. Moreover for the entomologist, the addition of the use of the microscope to their tool kit adds a whole new dimension to their study, revealing in finest detail the appearance and structure of these tiny creatures. This book reveals the basics of insect microscopy, explaining what equipment is needed and how to get the best out of it. Topics covered include insects and their relatives; trapping insects for study; dissection, slide mounting, and publishing your work. This fascinating guide to the basics of insect microscopy will make ideal material for study by the recreational microscopist and will be of great interest to science students and entomologists. Beautifully illustrated with 140 colour photographs.


Fluorescence Microscopy

Fluorescence Microscopy

Author: Ulrich Kubitscheck

Publisher: John Wiley & Sons

Published: 2017-03-27

Total Pages: 508

ISBN-13: 3527687726

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Zu dem Thema gibt es viele Publikationen, die von Experten für Experten geschrieben wurden. Dieses Buch wendet sich insbesondere an Studenten höherer Semester und Forscher, denen das Hintergrundwissen der Physik fehlt, um neuartige Verfahren der Fluoreszenzmikroskopie zu verstehen. Die zweite Auflage wartet mit neuen Kapiteln und einer erweiterten Einführung auf. Der Schwerpunkt liegt auf der hochauflösenden und Einzelmolekül-Mikroskopie. Jedes Kapitel wurde von einem anerkannten Experten des Fachgebiets geschrieben und sorgfältig überarbeitet, um so die Entwicklungen der letzten Jahre wiederzugeben.


Video Microscopy

Video Microscopy

Author: Shinya Inoue

Publisher: Springer Science & Business Media

Published: 2013-11-11

Total Pages: 600

ISBN-13: 1475769253

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Ever since television became practical in the early 1950s, closed-circuit television (CCTV) in conjunction with the light microscope has provided large screen display, raised image contrast, and made the images formed by ultraviolet and infrared rays visible. With the introduction of large-scale integrated circuits in the last decade, TV equipment has improved by leaps and bounds, as has its application in microscopy. With modem CCTV, sometimes with the help of digital computers, we can distill the image from a scene that appears to be nothing but noise; capture fluorescence too dim to be seen; visualize structures far below the limit of resolution; crispen images hidden in fog; measure, count, and sort objects; and record in time-lapsed and high-speed sequences through the light microscope without great difficulty. In fact, video is becoming indispensable for harnessing the fullest capacity of the light microscope, a capacity that itself is much greater than could have been envisioned just a few years ago. The time seemed ripe then to review the basics of video, and of microscopy, and to examine how the two could best be combined to accomplish these tasks. The Marine Biological Laboratory short courses on Analytical and Quantitative Light Microscopy in Biology, Medicine, and the Materials Sciences, and the many inquiries I received on video microscopy, supported such an effort, and Kirk Jensen of Plenum Press persuaded me of its worth.


Under the Microscope

Under the Microscope

Author: William J. Croft

Publisher: World Scientific

Published: 2006

Total Pages: 153

ISBN-13: 9810237812

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This is a brief history of the development of microscopy, from the use of beads and water droplets in ancient Greece, through the simple magnifying glass, to the modern compound microscope. The technology and optical theory are developed in a straightforward manner, and this leads to a description and explanation of the most modern technologies in electron microscopy, and scanning electron microscopy as well as the new scanning probe microscopies. A series of very interesting applications of the various microscopic techniques are described. The most recent pioneering techniques in near field and confocal optical microscope technologies are described and evaluated for their future importance.