Joanna Wilson and Gerhard May have assembled a collection of the key molecular biology protocols used in the analysis of Epstein-Barr virus (EBV), along with a series of valuable immunology, cell biology, and transgenic mouse protocols. These readily reproducible techniques include methods for gene expression with mini-EBV plasmids, for expression analysis by FISH, for EBV detection and quantitation, and for cell proliferation and death assays. In addition, there are EBV-based vectors, an up-to-date map of EBV, a comprehensive table of available latent protein antisera, and assays from in vitro to cell to organ to organism levels. Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating the life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures for EBV associated diseases, including several forms of human cancer.
The discovery of Epstein-Barr virus (EBV) by Epstein, Achong, and Barr, reported in 1964 (Lancet 1:702–703), was stimulated by Denis Burkitt’s rec- nition of a novel African childhood lymphoma and his postulation that an infectious agent was involved in the tumor’s etiology (Nature194:232–234, 1962). Since then, molecular and cellular biological and computational technologies have progressed by leaps and bounds. The advent of recombinant DNA technology opened the possibilities of genetic research more than most would have realized. Not only have the molecular tools permitted the analyses of viral mechanisms, but, importantly, they have formed the basis for discerning viral presence and, subsequently, viral involvement in an increasing number of diseases. Though in every field of science the search for further knowledge is likely to be a limitless phenomenon, the distinct goal in EBV research, namely, to gain sufficient insight into the viral–host interaction to be able to intercept the pathogenic process, is beginning to be realized. Epstein-Barr virus research has effectively entered the postgenomic era that began with the sequencing of the first strains, cloned in the mid to late 1980s.
The Epstein-Barr virus was discovered 15 years ago. Since that time an immense body of information has been accumu lated on this agent which has come to assume great signifi cance in many different fields of biological science. Thus, the virus has very special relevance in human medicine and oncology, in tumor virology, in immunology, and in mole cular virology, since it is the cause of infectious mononu cleosis and also the first human cancer virus, etiologically related to endemic Burkitt's lymphoma and probably to nasopharyngeal carcinoma. In addition, continuous human lymphoid cell lines initiated and maintained by the transform ing function of the virus genome provide a laboratory tool with wide and ever-growing applications. Innumerable papers on the Epstein-Barr virus have ap peared over recent years and reports of work with this agent now constitute a veritable flood. The present book provides the first and only comprehensive, authoritative over-view of all aspects of the virus by authors who have been the original and major contributors in their particular disciplines. A complete and up-to-date survey of this unique and important agent is thus provided which should be of great interest to experts, teachers, and students engaged in cancer research, virology, immunology, molecular biology, epide miology, and cell culture. Where topics have been dealt with from more than one of these viewpoints, some inevitable overlap and duplication has resulted; although this has been kept to a minimum, it has been retained in some places because of positive usefulness.
The application of recombinant DNA technology to the analysis of Epstein-Barr virus (EBV) is rapidly developing sufficient insight into the virus-host interaction, so that its role in disease pathology is now often discernible and can increasingly be interdicted. In Epstein-Barr Virus Protocols, Joanna Wilson and Gerhard May have assembled a collection of the key molecular biology protocols used in the analysis of Epstein-Barr virus, along with a series of valuable immunology, cell biology, and transgenic mouse protocols. Described in step-by-step detail by experts who use them regularly, these readily reproducible techniques include methods for gene expression with mini-EBV plasmids, for expression analysis by FISH, for EBV detection and quantitation, and for cell proliferation and death assays. In addition, the authors provide information on EBV-based vectors, an up-to-date map of EBV, a comprehensive table of available latent protein antisera, and assays from in vitro to cell to organ to organism levels. Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating the life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures for EBV-associated diseases, including several forms of human cancer.
This comprehensive account of the human herpesviruses provides an encyclopedic overview of their basic virology and clinical manifestations. This group of viruses includes human simplex type 1 and 2, Epstein–Barr virus, Kaposi's Sarcoma-associated herpesvirus, cytomegalovirus, HHV6A, 6B and 7, and varicella-zoster virus. The viral diseases and cancers they cause are significant and often recurrent. Their prevalence in the developed world accounts for a major burden of disease, and as a result there is a great deal of research into the pathophysiology of infection and immunobiology. Another important area covered within this volume concerns antiviral therapy and the development of vaccines. All these aspects are covered in depth, both scientifically and in terms of clinical guidelines for patient care. The text is illustrated generously throughout and is fully referenced to the latest research and developments.
The world's leading reference in hematopathology returns with this completely updated second edition. Authored by international experts in the field, it covers a broad range of hematologic disorders -- both benign and malignant -- with information on the pathogenesis, clinical and pathologic diagnosis, and treatment for each. Comprehensive in scope, it's a must-have resource for both residents and practicing pathologists alike. Authored by the chief architects of the WHO classification in neoplasms of hematopoietic and lymphoid tissue. Covers the newest diagnostic techniques, including molecular, immunohistochemical, and genetic studies. Confirm or challenge your diagnostic interpretations by comparing specimens to over 1,000 high-quality color images. Boasts detailed, practical advice from world leaders in hematopathology. Places an emphasis on pathologic diagnoses, including molecular and genetic testing. Updated with the most current WHO classifications of hematologic disease, including lymphoma and leukemia and peripheral T-cell lymphomas. Covers hot topics in hematopathology, such as the latest genetic insights into lymphoma and leukemia; the new nomenclature for myelodysplastic syndromes; new developments on the subject of Grey zone lymphoma; and much more.
From the Best Selling author Dr. Kasia Kines. Take your life back! For anyone suffering from Epstein-Barr Virus (EBV), chronic fatigue, autoimmune disorders, Infectious Mononucleosis, or other mystery illnesses, this is a must read!Do you feel lost and hopeless, living a life of illness with no answers? Do you feel unsupported by your medical team, left to suffer alone with no guidance? Are you searching for solutions to get your health and life back on track?Finally, a book that speaks to both the medical community and people inflicted by both mono and chronic conditions caused by EBV!The expanse of this pivotal book is far reaching - many people may not yet know they have been infected with EBV (commonly known as the "kissing disease"), but yet they struggle every day with chronic symptoms such as severe brain fog, debilitating fatigue, headaches, fever, chills, sore throat, body aches, and abdominal discomfort and can't seem to find a successful treatment solution.Eventually, some go on to develop autoimmune disorders, e.g. Lupus, Multiple Sclerosis, or Hashimoto's Thyroiditis and even cancer and are told that their immune system is simply confused and is now attacking itself, left with no solutions or hope for recovery.Alarmingly, chronic EBV infection is almost completely missed in medical practice, and as a result of never being tested, misinterpreting the test results, not being diagnosed, or being mis-diagnosed by their doctors, millions of people suffer debilitating conditions for many years with no answers.But this suffering can be avoided. And with this book, answers can be found.Author Dr. Kasia Kines is considered by her colleagues to be "one of the most brilliant, and importantly, clinically experienced functional nutritionists in the world." In Epstein-Barr Virus Solution, Dr. Kines provides a groundbreaking examination of EBV and not only how it causes chronic fatigue, autoimmune disorders, and even cancer but also how recovery is possible. This book was written in hopes that it will globally change the way EBV is understood, diagnosed, and treated.The deep journey into the existing research sheds light on the EBV virus for both the seasoned medical community and the layperson. Along with the validated research, Epstein-Barr Virus Solution presents practical, time-tested, and evidence-based clinical solutions for the testing and medical nutrition therapy for EBV, testing and lab interpretation, unique supplementation protocols, recovery strategies for stress, insomnia, fatigue, and toxicity, pantry and kitchen rehab for EBV, and delicious anti-EBV recipes. Through Dr. Kines' compassionate guidance, readers will feel confident to discuss their concerns with their doctor, to ask for testing, and to take their lives back from EBV. Epstein-Barr Virus Solution urges medical providers to hear their clients' concerns and provides the medical community with the research and insights needed to avoid misdiagnosis and common problems with testing for EBV.Epstein-Barr Virus Solution was made possible because of Dr. Kines' long-standing expertise as a functional clinical nutritionist who has dedicated her practice to working with clients inflicted by EBV. Dr. Kines tells readers in easy-to-understand language that recovery and hope for better health are possible through targeted nutrition and lifestyle changes. Readers are given a toolkit full of practical advice and guidance, including exclusive materials from Dr. Kines' life-changing 30-Day Detox Program that she has taught successfully for over 10 years.The research is out there, and Dr. Kasia Kines has found it, synthesized it, and utilized it to implement successful treatment protocols in her own clinic. Clients who had suffered years with chronic EBV infection symptoms are now recovering and living their lives.This book will provide those infected with EBV everything they need to finally take control of their health and take their life and happiness back.
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