Enzyme histochemical methods depend on visual identification of reaction products for the identification and localization of specific enzymes within tissue. This concise handbook is designed to give students, pathology, MLSO's and research workers an outline and handy lab reference to the methods which have become routine in research and hospital histopathological laboratories. The author discusses the most reliable and diagnostically important methods, and offers suggestions for further reading.
This book systematically illustrates theories and technologies in Histochemistry, including different kinds of enzymes, immunohistochemistry, polymerase chain reaction, related electron microscopic cytochemical techniques as well as the quantitative assay metrology. Abundant experiments as well as vivid images are demonstrated, making the book an essential reference for both graduate students and researchers in biochemistry.
During recent years enzyme histochemical reactions have increasingly been considered as important, the reason being that enzyme histo chemistry is now a well-established link between morphology and bio chemistry. The development of numerous new methods and in particular the improvement of existing techniques contributed to the expansion of enzyme histochemical reactions. Today, the use of these methods allows detailed insight into molecular processes of single cells and their constituents. The selection of a suitable method for enzyme histochemical investigations needs thorough knowledge and critical evaluation of the reactions de scribed for the histochemical demonstration of enzymes and introduced in laboratory practice. Often, it is difficult for scientists primarily concerned with the application of methods and for laboratory assistants to comment on the value of an enzyme histochemical reaction. Our book will serve as a guide in this respect. It contains the most important histochemical methods for the localization of enzymes, all of which were checked by the authors themselves. These methods were often modified and frequently used for numerous different investigations of healthy and diseased organs in basic research and in routine practice.
The Novartis Foundation Series is a popular collection of the proceedings from Novartis Foundation Symposia, in which groups of leading scientists from a range of topics across biology, chemistry and medicine assembled to present papers and discuss results. The Novartis Foundation, originally known as the Ciba Foundation, is well known to scientists and clinicians around the world.
This book systematically illustrates theories and technologies in Histochemistry, including different kinds of enzymes, immunohistochemistry, polymerase chain reaction, related electron microscopic cytochemical techniques as well as the quantitative assay metrology. Abundant experiments as well as vivid images are demonstrated, making the book an essential reference for both graduate students and researchers in biochemistry.
The functional analysis of macromolecular structures in tissues and cells has been greatly enhanced by advances in histochemistry and cytochemistry. Enzyme histochemistry is becoming particularly important as new methods succeed in demonstrating and quantifying the activity of key regulatory enzymes. The specificity, precision, and reproducibility of enzyme histochemical methods are constantly improving. This practical laboratory handbook contains a selection of the most important enzyme histochemical techniques currently available for light microscopy. The methods included here were chosen because of their reliability and specificity, and all are clearly detailed in easy-to-follow protocols. The introductory chapter provides a good theoretical background to enzyme histochemistry, and the book will be of interest to all researchers in cell biology, pathology, biochemistry, and cell physiology.
Histochemistry: An Explanatory Outline of Histochemistry and Biophysical Staining describes the histochemical staining of cells and tissues as a major tool applied in biological and medical investigations, both in basic research and in practical applications such as clinical diagnosis. The book may be considered as a guide to understanding the scientific basis of staining procedures and alternate actions to take when common methods do not proceed as expected. The first chapter gives general theoretical ideas from which most part of the book is largely organized around. As the book considers the arts and crafts making up the practice of histochemistry and biological staining, emphasis is given to the common physicochemical aspects of the technically diverse methodologies involved. Hence, the author has drawn ideas and information from physicochemically and biochemically related fields, such as chromatography, pharmacology, photography, tanning, and textile dyeing. The bulk of the book is structured around groups of practical procedures, such as fixation, staining with dyestuffs, metal impregnation, and selective extraction as a test of significance. Lastly, general essays on the usefulness of staining theories and on the problems arising from reagent impurities are given as examples. The text is suitable for students and researchers in the fields of physiology, biochemistry, and pharmacology. Clinical laboratory scientists and those involved in the life sciences and biotechnology will benefit from reading the book.
