Drosophila Cells in Culture
Drosophila Cells in Culture

Author: Guy Echalier

Publisher: Academic Press

Published: 2017-11-30

Total Pages: 259

ISBN-13: 0128097043

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Drosophila Cells in Culture, Second Edition, includes comprehensive coverage of cell lines, methods for creating cell lines, methods for genome engineering, and the use of cell lines for genome wide rNAi screens. This publication summarizes over thirty years of experience in the handling of in vitro cultured Drosophila cells alongside recent methods and functional screens. Early and experienced researchers studying drosophila in developmental biology, genetics, neuroscience, and across the biological and biomedical sciences will benefit from this expert knowledge. - Offers full coverage of cell lines and primary cultures - Provides a go-to resource for methods and studies completed with drosophila cells in culture - Presents a wide spectrum of experimental techniques

Drosophila Protocols
Drosophila Protocols

Author: William Sullivan

Publisher: CSHL Press

Published: 2000

Total Pages: 798

ISBN-13: 9780879695866

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This exceptional laboratory manual describes thirty-seven procedures most likely to be used in the next decade for molecular, biochemical, and cellular studies on Drosophila. They were selected after extensive consultation with the research community and rigorously edited for clarity, uniformity, and conciseness.The methods included permit investigation of chromosomes, cell biology, molecular biology, genomes, biochemistry, and development. Each protocol includes the basic information needed by novices, with sufficient detail to be valuable to experienced investigators. Each method is carefully introduced and illustrated with figures, tables, illustrations, and examples of the data obtainable. The book's appendices include key aspects of Drosophila biology, essential solutions, buffers, and recipes.An evolution of Michael Ashburner's 1989 classic Drosophila: A Laboratory Manual, this book is an essential addition to the personal library of Drosophila investigators and an incomparable resource for other research groups with goals likely to require fly-based technical approaches.

New Insights into Cell Culture Technology
New Insights into Cell Culture Technology

Author: Sivakumar Joghi Thatha Gowder

Publisher: BoD – Books on Demand

Published: 2017-05-10

Total Pages: 204

ISBN-13: 9535131338

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The book "New Insights into Cell Culture Technology" focuses on many advanced methods and techniques concerned with cell culture. The contributing authors have discussed various developments in cell culture methods, the application of insect cells for the efficient production of heterologous proteins, the expansion of human mesenchymal stromal cells for different clinical applications, the remote sensing of cell culture experiments and concepts for the development of cell culture bioprocess, continuous production of retroviral pseudotype vectors, and the production of oncolytic measles virus vectors for cancer therapy. This book is an original contribution of experts from different parts of the globe, and the in-depth information will be a significant resource for students, scientists, and physicians who are directly dealing with cells.["Culture" is essential for human life and also the life of a cell. - Sivakumar Gowder]

Insect Cell Culture Engineering
Insect Cell Culture Engineering

Author: Mattheus F. A. Goosen

Publisher: CRC Press

Published: 2020-07-24

Total Pages: 260

ISBN-13: 1000105660

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Consolidating and expanding current, fundamental notions of virology and animal cell cultivation, this practical reference examines the development of insect cell culture techniques for the production of recombinant proteins and insect pathogenic viruses.;Resolving on-the-job problems such as sparging cell damage and reduced infectivity cells, Insect Cell Culture Engineering: includes special introductory material as well as background information on insect pathogenic viruses, the molecular biology of baculoviruses and bioreactor design; offers advice on how to save time when deciding which insect cell line, bioreactor and medium to exploit; discusses the preparation of mathematical modelling in animal cell culture; addresses the concerns associated with insect cell immobilization and the use of serum-free culture media; provides insights into the protective effects of polymer additives and insect cell gene expression in pharmaceutical research; and analyzes process scale-up and reactor design.;Bridging the gap between laboratory research and pilot plant scale insect culture/baculovirus technology, Insect Cell Culture Engineering is designed as a reference for biochemical and bioprocess engineers, bioprocess technologists, biochemists, molecular and cell biologists, microbiologists, and upper-level undergraduate and graduate students in these disciplines.

Muscle Development in Drosophilia
Muscle Development in Drosophilia

Author: Helen Sink

Publisher: Springer Science & Business Media

Published: 2006-02-02

Total Pages: 224

ISBN-13: 9780387300535

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The different aspects of muscle development are considered from cellular, molecular and genetic viewpoints, and the text is supported by black/white and color illustrations. The book will appeal to those studying muscle development and muscle biology in any organism.

Germline Stem Cells
Germline Stem Cells

Author: Michael Buszczak

Publisher: Humana

Published: 2016-10-13

Total Pages: 0

ISBN-13: 9781493940158

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This second edition provides updated and new chapters on selected genetic, molecular, biochemical, and cell biological techniques. Chapter’s guide readers through methods and principles on primordial germ cells and germline stem cells, however many of these principles can be applied to different types of adult stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Germline Stem Cells: Second Edition aims to present the new findings and techniques that have developed since the first edition.

Understanding the Genome
Understanding the Genome

Author: Editors of Scientific American

Publisher: Hachette UK

Published: 2002-06-01

Total Pages: 160

ISBN-13: 075952758X

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Drawn from the pages of Scientific American and collected here for the first time, this work contains updated and condensed information, made accessible to a general popular science audience, on the subject of understanding the genome.

Fly Pushing
Fly Pushing

Author: Ralph J. Greenspan

Publisher: CSHL Press

Published: 2004

Total Pages: 212

ISBN-13: 9780879697112

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A second edition of the classic handbook has become a standard in the Drosophila field. This edition is expanded to include topics in which classical genetic strategies have been augmented with new molecular tools. Included are such new techniques as homologous recombination, RNAi, new mapping techniques, and new mosaic marking techniques.