Cooperativity and Regulation in Biochemical Processes
Cooperativity and Regulation in Biochemical Processes

Author: Arieh Y. Ben-Naim

Publisher: Springer Science & Business Media

Published: 2013-03-09

Total Pages: 358

ISBN-13: 147573302X

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This is the first book that attempts to study the origin of cooperatvity in binding systems from the molecular point of view. The molecular approach provides a deeper insight into the mechanism of cooperativity and regulation, than the traditional phenomenological approach. This book uses the tools of statistical mechanics to present the molecular theory of cooperativity. Cooperativity is used in a variety of processes-such as loading and unloading of oxygen at relatively small pressure differences; maintaining an almost constant concentration of various compounds in living cells; and switching on and off the reading of genetic information. This book may be used as a textbook by graduate students in Chemistry, Biochemistry and Biophysics, and will also be of interest to researchers in theoretical biochemistry.

Enzyme Catalysis and Regulation
Enzyme Catalysis and Regulation

Author: Gorden Hammes

Publisher: Elsevier

Published: 2012-12-02

Total Pages: 276

ISBN-13: 032315817X

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Enzyme Catalysis and Regulation is an introduction to enzyme catalysis and regulation and covers topics ranging from protein structure and dynamics to steady-state enzyme kinetics, multienzyme complexes, and membrane-bound enzymes. Case studies of selected enzyme mechanisms are also presented. This book consists of 11 chapters and begins with a brief overview of enzyme structure, followed by a discussion on methods of probing enzyme structure such as X-ray crystallography and optical spectroscopy. Kinetic methods are then described, with emphasis on the general principles of steady-state and transient kinetics. The chemical principles involved in enzyme catalysis are also discussed, and case studies of a few well-documented enzymes are presented. The regulation of enzyme activity is analyzed from a nongenetic viewpoint, with particular reference to binding isotherms and models for allosterism. Two particular enzymes, aspartate transcarbamoylase and phosphofructokinase, are used as examples of well-studied regulatory enzymes. The last two chapters focus on multienzyme complexes and membrane-bound enzymes. This monograph is intended for graduate students, advanced undergraduates, and research workers in molecular biology and biochemistry.

Control of Metabolic Processes
Control of Metabolic Processes

Author: Athel Cornish-Bowden

Publisher: Springer

Published: 1990-08-31

Total Pages: 480

ISBN-13:

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THIS BOOK collects together papers given at a NATO Advanced Research Workshop held at Il Ciocco (Lucca), Italy, from the 9th to the 15th April, 1989. It sets out to present the current state of understanding of the principles governing the way fluxes and concentrations are maintained and controlled in metabolic systems. Although this is a topic that has held the interest of biochemists for many years, it is only quite recently that the methods of analysing the kinetics of multi-enzyme pathways developed over the past two decades have come to be widely discussed or applied experimentally. Many biochemists remain sceptical that the new methods offer a real advance (except in complexity) over the landmark discoveries of the 1950s and 1960s relating to inhibition of enzymes at branch-points by the end products of metabolic pathways, and the interpretation of allosteric effects and cooperativity. Even those who have become convinced that the classical ideas provide only the starting point for understanding metabolic control have been by no means unanimous in their assess ment of the direction in which one should advance. In this book we have tried to include all of the current points of view, including the view that the classical theories tell us all that we need to know. We have not seen it as our role as editors to paper over the cracks that exist and to pretend that we can speak to the world with one voice.

Regulation of Tissue Oxygenation, Second Edition
Regulation of Tissue Oxygenation, Second Edition

Author: Roland N. Pittman

Publisher: Biota Publishing

Published: 2016-08-18

Total Pages: 117

ISBN-13: 1615047212

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This presentation describes various aspects of the regulation of tissue oxygenation, including the roles of the circulatory system, respiratory system, and blood, the carrier of oxygen within these components of the cardiorespiratory system. The respiratory system takes oxygen from the atmosphere and transports it by diffusion from the air in the alveoli to the blood flowing through the pulmonary capillaries. The cardiovascular system then moves the oxygenated blood from the heart to the microcirculation of the various organs by convection, where oxygen is released from hemoglobin in the red blood cells and moves to the parenchymal cells of each tissue by diffusion. Oxygen that has diffused into cells is then utilized in the mitochondria to produce adenosine triphosphate (ATP), the energy currency of all cells. The mitochondria are able to produce ATP until the oxygen tension or PO2 on the cell surface falls to a critical level of about 4–5 mm Hg. Thus, in order to meet the energetic needs of cells, it is important to maintain a continuous supply of oxygen to the mitochondria at or above the critical PO2 . In order to accomplish this desired outcome, the cardiorespiratory system, including the blood, must be capable of regulation to ensure survival of all tissues under a wide range of circumstances. The purpose of this presentation is to provide basic information about the operation and regulation of the cardiovascular and respiratory systems, as well as the properties of the blood and parenchymal cells, so that a fundamental understanding of the regulation of tissue oxygenation is achieved.

Biophysics DeMYSTiFied
Biophysics DeMYSTiFied

Author: Daniel Goldfarb

Publisher: McGraw Hill Professional

Published: 2010-12-06

Total Pages: 400

ISBN-13: 0071633650

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Learn BIOPHYSICS without expending a lot of ENERGY! Interested in unraveling the physics of living things? Here's your starting point. Biophysics Demystified is the fast and easy way to understand this fascinating topic. Written in a step-by-step format, this practical guide begins with an introduction to the science of biophysics, covering biophysical techniques and applications. Next, you'll learn the principles of physics, biology, and chemistry required to understand biophysics, including free energy, entropy, and statistical mechanics. Biomolecules and the forces that influence their structure and conformation are also covered, as are protein, nucleic acid, and membrane biophysics. Detailed examples and concise explanations make it easy to understand the material, and end-of-chapter quizzes and a final exam help reinforce key concepts. It's a no-brainer! You'll get: Molecular, subcellular, physiological, anatomical, and environmental biophysics The laws of thermodynamics as they apply to biophysical systems Forces affecting conformation in biological molecules The composition and structure of carbohydrates, lipids, proteins, and nucleic acids The fluid mosaic model Simple enough for a beginner, but challenging enough for an advanced student, Biophysics Demystified makes this interdisciplinary subject easy to master.

Structure and Mechanism in Protein Science
Structure and Mechanism in Protein Science

Author: Alan Fersht

Publisher: World Scientific Publishing Company

Published: 2017

Total Pages: 0

ISBN-13: 9789813225190

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The three-dimensional structure of proteins -- Chemical catalysis -- The basic equations of enzyme kinetics -- Measurement and magnitude of individual rate constants -- The pH dependence of enzyme catalysis -- Practical methods for kinetics and equilibria -- Detection of intermediates in enzymatic reactions -- Stereochemistry of enzymatic reactions -- Active-site-directed and enzyme-activated irreversible inhibitors : "affinity labels" and "suicide inhibitors" -- Conformational change, allosteric regulation, motors, and work -- Forces between molecules, and binding energies -- Enzyme-substrate complementarity and the use of binding energy in catalysis -- Specificity and editing mechanisms -- Recombinant DNA technology -- Protein engineering -- Case studies of enzyme structure and mechanism -- Protein stability -- Kinetics of protein folding -- Folding pathways and energy landscapes.

Energetics of Biological Macromolecules, Part D
Energetics of Biological Macromolecules, Part D

Author: Jo M. Holt

Publisher: Elsevier

Published: 2004-04-02

Total Pages: 315

ISBN-13: 0080497179

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This volume focuses on the cooperative binding aspects of energetics in biological macromolecules. Methodologies such as NMR, small-angle scattering techniques for analysis, calorimetric analysis, fluorescence quenching, and time resolved FRET measurements are discussed.*Methods for Evaluating Cooperativity in a Dimeric Hemoglobin*Multiple-Binding of Ligands to a Linear Biopolymer*Fluorescence Quenching Methods to Study Protein-Nucleic Acid Interactions*Linked Equilibria in Biotin Repressor Function: Thermodynamic, Structural and Kinetic Analysis

Physical Biology of the Cell
Physical Biology of the Cell

Author: Rob Phillips

Publisher: Garland Science

Published: 2012-10-29

Total Pages: 1089

ISBN-13: 1134111584

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Physical Biology of the Cell is a textbook for a first course in physical biology or biophysics for undergraduate or graduate students. It maps the huge and complex landscape of cell and molecular biology from the distinct perspective of physical biology. As a key organizing principle, the proximity of topics is based on the physical concepts that