Computer-assisted Method Development for High-performance Liquid Chromatography
Computer-assisted Method Development for High-performance Liquid Chromatography

Author: Joseph L. Glajch

Publisher: Elsevier Publishing Company

Published: 1990

Total Pages: 714

ISBN-13:

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This book deals with the use of the computer as an aid in selecting adequate or optimum conditions for a given analytical separation. Originally published as Volume 485 of the Journal of Chromatography, it has now been reprinted in book form, since the information is so useful that many chromatographers want a copy readily available in the lab. An extensive Introduction is added to the book edition. This surveys the field and refers to the pages where particular items are discussed in the book. The addition of a Glossary of Terms, an Author Index and a Subject Index make this book an invaluable source of easily consulted information for the practising chromatographer. For the purpose of this book, computer-assisted method development will be limited to specific procedures which are intended to be used with a computer - rather than their manually applied precursors. In that sense, the subject can be considered to have begun around 1980. The ongoing, intense research activity into various forms of computer assisted HPLC method development provides the assurance that this approach can really assist the practical chromatographer working in an industrial laboratory.

Software-assisted Method Development In High Performance Liquid Chromatography
Software-assisted Method Development In High Performance Liquid Chromatography

Author: Szabolcs Fekete

Publisher: World Scientific

Published: 2018-08-01

Total Pages: 364

ISBN-13: 1786345471

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'The book is a useful contribution in the field of HPLC, and may represent a valuable tool for chromatography practitioners in different fields, as well as teachers and instructors. The 12 chapters provide comprehensive insights of current day retention and resolution modelling in HPLC, and its applications for small and large molecule analysis. It may be a useful reference for specialists in pharmaceuticals but not limited to … It may be a valuable resource to assist scientists involved in method development, aiming to achieve the best results with reduced costs, time, and efforts.'Analytical and Bioanalytical ChemistryThis handbook gives a general overview of the possibilities in recent developments in chromatographic retention modeling. As a result of the latest developments in modeling software, several new features are now accessible, opening a new level in HPLC method development.Many of these current possibilities in software assisted liquid chromatographic method modeling for analytical purposes are presented. Several modes of chromatography, including Reversed-Phase Liquid Chromatography (RPLC), Ion Exchange Chromatography (IEX), Hydrophobic Interaction Chromatography (HIC), and Hydrophilic Interaction Liquid Chromatography (HILIC) are explained in detail. For all these chromatographic modes, the most important variables for tuning retention and selectivity are exposed.Beside the industrial and practical benefits of retention modeling, the possibilities in teaching and education are also illustrated. Finally, numerous representative industrial examples are shown, to highlight the benefits, time and cost savings offered by state-of-the-art software assisted HPLC method development.

Practical HPLC Method Development
Practical HPLC Method Development

Author: Lloyd R. Snyder

Publisher: John Wiley & Sons

Published: 2012-12-03

Total Pages: 665

ISBN-13: 1118591518

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This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.

Dry Lab® Modeling Computer Assisted Method Develoment and Robustness Validation for the Seperation [sic] of Nine Pharmaceutical Active Ingredients Using Gradient Elution Reversed-phase Separation
Dry Lab® Modeling Computer Assisted Method Develoment and Robustness Validation for the Seperation [sic] of Nine Pharmaceutical Active Ingredients Using Gradient Elution Reversed-phase Separation

Author: Shaista Khan

Publisher:

Published: 2017

Total Pages: 280

ISBN-13:

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"High performance liquid chromatography (HPLC) method development is a technique for drug analysis that is a time consuming process. To prevent the need for a trial and error process, one can use a computer assisted method. In this thesis, we report about the use of Dry Lab to develop and optimize reverse phase liquid chromatography method to separate the following 10 drugs: 1. Clonidine 2. Gatifloxacin 3. Hydrochlorothizaide 4. Triprolidine 5. Fluoconozole 6. Trazodone 7. Pantaeprozole 8. X-Drug 9. Rivarzaban and 10. Ketorolac. Chromatographic separation of mixture of the above drugs was achieved by applying Gradientelution technique at a flow rate of 1.0 ml/min. The organic solvent of choice was ACN and the mobile phase was optimized at a composition of 30% ACN and 70% buffered deionized water. The best suited buffer was a monobasic potassium phosphate salt at a concentration of 25 mm and the mobile phase pH was set 2.88 pH. The separation was performed on a reversed phase, silica based, C8column(250x4.6mm) made by Waters Corporation®, with a particle size of 5 μm. The column temperatures were controlled at 35°C and 55°C; the UV absorption wavelength initially was set at 260, 267 and 290nm, and finally 260nm. The injected volume of a mixture of the above drugs was 10 μl and the total run time for the method was 24 minutes. The method was validated within ICH and FDA guidelines."--

Introduction to Modern Liquid Chromatography
Introduction to Modern Liquid Chromatography

Author: Lloyd R. Snyder

Publisher: John Wiley & Sons

Published: 2011-09-20

Total Pages: 831

ISBN-13: 1118210395

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The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.

Preparative Chromatography
Preparative Chromatography

Author: H. Schmidt-Traub

Publisher: John Wiley & Sons

Published: 2012-09-27

Total Pages: 545

ISBN-13: 3527649301

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Completely revised and substantially extended to reflect the developments in this fast-changing field. It retains the interdisciplinary approach that elegantly combines the chemistry and engineering involved to describe the conception and improvement of chromatographic processes. It also covers recent advances in preparative chromatographic processes for the separation of "smaller" molecules using standard laboratory equipment as well as the detailed conception of industrial chemical plants. The increase in biopharmaceutical substances is reflected by new and revised chapters on different modifications of continuous chromatography as well as ion-exchange chromatography and other separation principles widely used in biochromatography. Following an introductory section on the history of chromatography, the current state of research and the design of chromatographic processes, the book goes on to define the general terminology. There then follow sections on stationary phases, selection of chromatographic systems and process concepts. A completely new chapter deals with engineering and operation of chromatographic equipment. Final chapters on modeling and determination of model parameters as well as model based design, optimization and control of preparative chromatographic processes allow for optimal selection of chromatographic processes. Essential for chemists and chemical engineers in the chemical, pharmaceutical, and food industries.

High-Performance Gradient Elution
High-Performance Gradient Elution

Author: Lloyd R. Snyder

Publisher: John Wiley & Sons

Published: 2007-01-09

Total Pages: 496

ISBN-13: 0470055510

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Gradient elution demystified Of the various ways in which chromatography is applied today, few have been as misunderstood as the technique of gradient elution, which presents many challenges compared to isocratic separation. When properly explained, however, gradient elution can be less difficult to understand and much easier to use than often assumed. Written by two well-known authorities in liquid chromatography, High-Performance Gradient Elution: The Practical Application of the Linear-Solvent-Strength Model takes the mystery out of the practice of gradient elution and helps remove barriers to the practical application of this important separation technique. The book presents a systematic approach to the current understanding of gradient elution, describing theory, methodology, and applications across many of the fields that use liquid chromatography as a primary analytical tool. This up-to-date, practical, and comprehensive treatment of gradient elution: * Provides specific, step-by-step recommendations for developing a gradient separation for any sample * Describes the best approach for troubleshooting problems with gradient methods * Guides the reader on the equipment used for gradient elution * Lists which conditions should be varied first during method development, and explains how to interpret scouting gradients * Explains how to avoid problems in transferring gradient methods With a focus on the use of linear solvent strength (LSS) theory for predicting gradient LC behavior and separations by reversed-phase HPLC, High-Performance Gradient Elution gives every chromatographer access to this useful tool.