Characterization of Genes Involved in SUMOylation During Embryogenesis in Rainbow Trout (Oncorhynchus Mykiss)
Author: Xiaowei Zang
Publisher:
Published: 2013
Total Pages:
ISBN-13:
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Author: Xiaowei Zang
Publisher:
Published: 2013
Total Pages:
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DOWNLOAD EBOOKAuthor: Bih-Ying Yang
Publisher:
Published: 1997
Total Pages: 308
ISBN-13:
DOWNLOAD EBOOKAuthor: Bih-Ying Yang
Publisher:
Published: 1997
Total Pages: 0
ISBN-13:
DOWNLOAD EBOOKAuthor: Grant David Trobridge
Publisher:
Published: 1996
Total Pages: 264
ISBN-13:
DOWNLOAD EBOOKAuthor: Kathy A. Mangold
Publisher:
Published: 1989
Total Pages: 268
ISBN-13:
DOWNLOAD EBOOKThe rainbow trout (Oncorhynchus mykiss) model of chemical carcinogenesis is becoming increasingly important as a supplement to rodent studies. However, much of the molecular biology of the carcinogenic response is still unknown in the trout model. The ras gene family has been implicated in the tumorigenesis of both spontaneous and chemically-induced tumors in mammals. This study is the first to characterize a ras proto-oncogene in rainbow trout. To accomplish this, the ras gene sequence was amplified in vitro by using polymerase chain reaction (PCR). Two synthetic and degenerative oligonucleotide sequences based on a consensus mammal/goldfish ras sequence were used as primers in the PCR procedure. An 800 base pair (bp) sequence was amplified from trout genomic DNA and hybridized with a human c-Ha-ras sequence. The initial amplifications of trout liver cDNA using the PCR procedure with the synthetic ras primers resulted in a single product of approximately 216 bps. However, this amplified "trout" 216 by product was subsequently shown to be an artifact of carryover from a human Ki-ras plasmid. Carryover is a common problem found in many laboratories involved with the PCR procedure, and extensive precautions were used to eliminate the problem in our laboratories. The 800 by PCR product was cloned and sequenced using Taq polymerase. RT-8, a clone containing the 800 bp insert, was shown to have 91% homology to the first two exons of mammalian c-Ha-ras gene and lesser homology to other ras genes. Amplification of trout liver cDNA using specific primers based on the RT-8 sequence resulted in the amplification of sequences identical to the sequence of the RT-8 insert without an intron, as well as unique sequences, which may represent additional trout ras genes. The PCR procedure was modified to identify sequence information immediately 3' of the known trout ras sequence. Partial sequences of at least two different trout ras genes are presented. With this new information, analysis of DNA sequence information from chemically initiated tumors may elucidate the role activation of ras genes plays in the trout model of carcinogenesis.
Author: Jonathan W. Stoddard
Publisher:
Published: 2005
Total Pages: 86
ISBN-13:
DOWNLOAD EBOOKAuthor: Lei Wang
Publisher:
Published: 2009
Total Pages:
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DOWNLOAD EBOOKAuthor: Caroline Sue Tracy Cheng
Publisher:
Published: 2010
Total Pages: 192
ISBN-13:
DOWNLOAD EBOOK"The effects of aquatic toxicants on fish growth and development have been well-documented, in contrast to the changes in gene expression that necessarily precede them. Many of these toxicants are exogenous compounds that possess the ability to mimic steroid hormones or inhibit normal endocrine functions, which are known as endocrine disrupting compounds (EDCs). The Law laboratory previously obtained mRNA expression data indicating that exposure to aquatic effluents from a combined news/kraft pulp and paper mill changed the expression of genes associated with EDC exposure in the livers of fathead minnows (Pimephales promelas). Societal desire to reduce the use of animals in toxicity testing has encouraged the development and use of in vitro systems, including vertebrate cell models. In this study, the gene expression of the rainbow trout (Oncorhynchus mykiss) liver cell line RTL-W1 and the fathead minnow liver cell line FHM-L was examined following effluent exposure. In the RTL-W1 and FHM-L cell lines, 24 h exposure to effluents was sufficient for assessing changes in gene expression, with maximum changes observed between 4 and 6 h after exposure.
Author: Hans Cheng
Publisher: Frontiers Media SA
Published: 2021-12-20
Total Pages: 332
ISBN-13: 2889718298
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Publisher:
Published: 2004
Total Pages: 2036
ISBN-13:
DOWNLOAD EBOOKVols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.