Cell Separation
Cell Separation

Author: Thomas G. Pretlow

Publisher: Academic Press

Published: 2014-05-10

Total Pages: 344

ISBN-13: 1483219399

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Cell Separation: Methods and Selected Applications, Volume 2 provides information pertinent to the design and application of methods for the separation of cells. This book covers a variety of topics, including thyroid structure and function, procurement of eosinophils, elutriation, stromal cells, isolation of epithelial cells, and separation of cells. Organized into 15 chapters, this volume begins with an overview of the structure of thyroid parenchyma and the properties of endocrine parafollicular cells. This text then summarizes the various aspects of the separation methodology to assist investigators with the use of elutriation. Other chapters describe the methods that are useful for the separation of stromal cells from tumors. This book discusses as well the types of methods that are available for the purification of cells from tumors. The final chapter deals with the biological significance of the erythroblastic nest. This book is a valuable resource for cell biologists, experimental oncologists, hematologists, immunologists, and endocrinologists.

Human Naïve Pluripotent Stem Cells
Human Naïve Pluripotent Stem Cells

Author: Peter Rugg-Gunn

Publisher: Humana

Published: 2021-12-07

Total Pages: 288

ISBN-13: 9781071619070

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This volume provides readers with a comprehensive collection of methods to guide them on how to generate, characterize, and use naïve human pluripotent stem cells (hPSCs). The chapters in this book cover topics such as three predominant routes to generate naïve hPSC lines; methods to differentiate naïve hPSCs into specialized cell types; and techniques to characterize naïve hPSCs using key molecular landmarks that benchmark and quality control the cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Human Naïve Pluripotent Stem Cells: Methods and Protocols is a valuable resource for novice and expert researchers who are looking to learn more or expand their research in this developing field.

TRAC: Trends in Analytical Chemistry
TRAC: Trends in Analytical Chemistry

Author: C. J. W. Brooks

Publisher: Elsevier

Published: 2013-09-03

Total Pages: 301

ISBN-13: 1483274594

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Trends in Analytical Chemistry, Volume 5 focuses on the advancements of processes, technologies, automation, and applications of analytical chemistry. The selection first offers information on graphics programming for the IBM PC using FORTRAN, PASCAL, and C, including graphics hardware system software, assembly language routines, and high level interface. The text then elaborates on the place of affinity chromatography in the production and purification of biomolecules from cultured cells and zone electrophoresis in open-tubular capillaries. Discussions focus on column and instrument design, applications, affinity chromatography in protein production from cells, and economic aspects of production and purification of proteins from cell cultures. The manuscript takes a look at polarographic and voltammetric techniques and their application to the determination of vitamins and coenzymes and activation analysis with charged particles. Topics include accelerators, principle of charged particle activation analysis, and applications. The text then examines the development of microbiological and immunological assays for antibiotics and the use of computer system for a small analytical research laboratory. The book is a dependable reference for readers interested in the trends in analytical chemistry.