Bone Research Protocols
Bone Research Protocols

Author: Miep H. Helfrich

Publisher: Springer Science & Business Media

Published: 2008-02-01

Total Pages: 444

ISBN-13: 1592593666

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The last decade has seen a tremendous advance in our understanding of bone biology. The genes responsible for the majority of rare inherited bone disorders have been identified and much progress has been made in the identification of genes in polygenic disorders such as Paget’s disease and complex multigene diseases such as osteoporosis. Transgenic technology has identified further genes, sometimes unexpectedly, with profound effects on bone. This wealth of new genetic information will undoubtedly lead to extensive cell biological studies to understand the mechanisms by which these gene products affect bone mass and bone strength. In Bone Research Protocols a catalogue of protocols has been assembled to perform such mechanistic studies. In the tradition of the Methods in Molecular Medicine series, the chapters are practical laboratory protocols that should enable the reader to carry out the techniques from scratch. To our knowledge this is the first time such a truly practical manual on well-established bone methods has been assembled, and this volume aims to be complementary to and follow on from the more theoretical Methods in Bone Biology, edited by Arnett and Henderson (1).

Clinical Trials in Osteoporosis
Clinical Trials in Osteoporosis

Author: Derek Pearson

Publisher: Springer Science & Business Media

Published: 2002

Total Pages: 280

ISBN-13: 9781852332297

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Clinical Trials in Osteoporosisis a practical handbook on clinical trials in the growing field of osteoporosis. Topics covered include study design, technical issues, data collection, quality assurance, data analysis and presentation. It aims to take the user through the process step-by-step from start to finish, also providing a background on regulatory guidelines, ethical implications, endpoints, current therapies and the ideal drug to use. There are no other books at present that specifically address the issue of clinical trials in osteoporosis. A number of issues dealt with in this book have been brought together in one publication for the first time. Clinical Trials in Osteoporosisis intended to serve as a practical manual for clinicians and scientists coming to the subject new and to provide a standard for existing centers to measure themselves against.

Osteoporosis and Osteoarthritis
Osteoporosis and Osteoarthritis

Author: Andre J. van Wijnen

Publisher: Humana

Published: 2020-12-07

Total Pages: 302

ISBN-13: 9781071609880

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This detailed second edition provides key updates on well-established methods as well as coverage of recently emerging sophisticated techniques and methods that are essential for an in-depth and state-of-the-art understanding of skeletal development and homeostasis and the pathological mechanisms that cause skeletal degeneration. The first half of the book examines cell and molecular biological aspects of osteoarthritis and osteoporosis, while the remainder of the collection focuses on in vivo models of skeletal tissue injury, degeneration, and repair. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Osteoporosis and Osteoarthritis, Second Edition aims to assist investigators concerned with research topics broadly related to osteoporosis, osteoarthritis, intervertebral disc degeneration, as well as other musculoskeletal disorders.

Rebuild Your Bones
Rebuild Your Bones

Author: Mira Calton, CN

Publisher: Rodale Books

Published: 2019-09-17

Total Pages: 338

ISBN-13: 1635653738

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Your first step on the path to total bone health An osteoporosis diagnosis can feel like a debilitating life sentence—one that leaves you feeling stuck with a future of prescription drugs that only might keep the condition from worsening. Mira Calton, CN and Jayson Calton, PhD have discovered a better way to prevent and even reverse the disease through the power of micronutrient therapy. The secret to building strong bones lies in the right combination of micronutrients— the vitamins, minerals, essential fatty acids, and amino acids in the foods we eat and supplements we take. In Rebuild Your Bones, the Caltons reveal how our dietary, lifestyle, and even supplementation routines may be depleting these essential micronutrients, and share the 40 healing habits scientifically proven to build stronger bones. They also provide an easy-to-follow plan to reverse these effects, including recipes and meal plans, exercise advice, and supplement recommendations. If you’re looking for a pharmaceutical-free way to restore your bone health, look no further—this is the definitive guide to safely and naturally stave off osteoporosis and reclaim your health.

The Human Bone Manual
The Human Bone Manual

Author: Tim D. White

Publisher: Elsevier

Published: 2005-11-08

Total Pages: 485

ISBN-13: 0080488994

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Building on the success of their previous book, White and Folkens' The Human Bone Manual is intended for use outside the laboratory and classroom, by professional forensic scientists, anthropologists and researchers. The compact volume includes all the key information needed for identification purposes, including hundreds of photographs designed to show a maximum amount of anatomical information. - Features more than 500 color photographs and illustrations in a portable format; most in 1:1 ratio - Provides multiple views of every bone in the human body - Includes tips on identifying any human bone or tooth - Incorporates up-to-date references for further study

Handbook of Histology Methods for Bone and Cartilage
Handbook of Histology Methods for Bone and Cartilage

Author: Yuehuei H. An

Publisher: Springer Science & Business Media

Published: 2003-05-01

Total Pages: 582

ISBN-13: 1592594174

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Histotechnology and histomorphometry are the major methodologies in bone and cartila- related research. Handbook of Histology Methods for Bone and Cartilage is an outgrowth of the editors’ own quest for information on bone and cartilage histology and histomorphometry. It is designed to be an experimental guide for personnel who work in the areas of basic and clinical bone and cartilage, orthopedic, or dental research. It is the first inclusive and organized reference book on histological and histomorphometrical techniques on bone and cartilage specimens. The topic has not previously been covered adequately by any existing books in the field. Handbook of Histology Methods for Bone and Cartilage has six major parts and is designed to be concise as well as inclusive, and more practical than theoretical. The text is simple and straightforward. Large numbers of tables, line drawings, and micro- or macro-photographs, are used to help readers better understand the content. Full bibliographies at the end of each chapter guide readers to more detailed information. A book of this length cannot discuss every method for bone and cartilage histology that has been used over the years, but it is hoped that major methods and their applications have been included.

Stem Cell Protocols
Stem Cell Protocols

Author: Ivan N. Rich

Publisher: Humana Press

Published: 2014-11-11

Total Pages: 0

ISBN-13: 9781493917846

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This volume presents up-to-date methods that allow primary stem cells from a variety of sources to be isolated, cultured in vitro, detected and measured for specific applications. These applications range from those in basic, stem cell and veterinary research to toxicology, cellular therapy and regenerative medicine. There is a slight bias towards the blood-forming system as more is known about the blood-forming or hematopoietic system than any other primary stem cell system. These unique properties and characteristics are discussed and examined, mostly at the cellular level and in detail in this book. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Stem Cell Protocols provides novices with the fundamentals necessary to develop new technologies necessary for basic and clinical research in the future, and will aid professionals in finding new methodologies to provide a wider viewpoint and an even greater scope for their own research.

B Cell Protocols
B Cell Protocols

Author: Hua Gu

Publisher: Springer Science & Business Media

Published: 2008-02-04

Total Pages: 294

ISBN-13: 1592597963

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B-lymphocyte development and function remains an exciting area of research for those interested in the physiology and pathology of the immune system in higher animals. While recent advances in genetics and cellular and molecular biology have provided a large spectrum of powerful new experimental tools in this field, it is both time consuming and often very difficult for a student or just any bench-side worker to identify a reliable experimental protocol in the ocean of the literature. The aim of B Cell Protocols is to provide a collection of diverse protocols ranging from the latest inventions and applications to some classic, but still frequently used methods in B-cell biology. The authors of the various chapters are all highly qualified scientists who are either the inventors or expert users of these methods. Their extensive experience in mastering a particular method provides not only the step-by-step details of a reproducible protocol, but also useful troubleshooting tips that readers will appreciate in their daily work. We hope that this book will be helpful for both beginning and experienced researchers in the field in designing or modifying an experimental approach, and exploring a biological question from multiple angles.

Craniofacial Development
Craniofacial Development

Author: Sebastian Dworkin

Publisher:

Published: 2022

Total Pages: 313

ISBN-13: 9781071618479

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This volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (c. elegans, zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant and in vivo cell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression using in-situ hybridisation and single-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such as CRISPR/Cas9-mediated deletion. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Craniofacial Development: Methods and Protocols aims to be a guide in the field of craniofacial development for senior and new researchers looking to expand their existing research programs to encompass novel techniques. .