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Author: Margarida Bastos
Publisher: CRC Press
Published: 2016-06-22
Total Pages: 412
ISBN-13: 148224666X
DOWNLOAD EBOOKConnecting past, present, and future instrument development and use, Biocalorimetry: Foundations and Contemporary Approaches explores biocalorimetry's history, fundamentals, methodologies, and applications. Some of the most prominent calorimeter developers and users share invaluable personal accounts of discovery, discussing innovative techniques a
Author: John E. Ladbury
Publisher: John Wiley & Sons
Published: 2005-07-15
Total Pages: 276
ISBN-13: 0470011114
DOWNLOAD EBOOKOver the last decade, high-sensitivity calorimetry has developed from a specialist method used mainly by dedicated experts to a major, commercially available tool in the arsenal directed at understanding molecular interactions and stability. Calorimeters have now become commonplace in bioscience laboratories. As a result, the number of those proficient in experimentation in this field has risen dramatically, as has the range of experiments to which these methods have been applied. Applications extend from studies in small molecule and solvent biophysics, through drug screening to whole cell assays. The technology has developed to include higher levels of sensitivity (and hence smaller sample size requirements) and a drive towards high-throughput technology, creating a very large user base in both academia and the pharmaceutical industry. This book is a fully revised and updated edition of the successful Biocalorimetry: Applications of Calorimetry in the Biological Sciences, published in 1998. Since then, there have been many advances in the instrumentation as well as in its applications and methodology. There are general chapters highlighting the usage of the isothermal titration calorimeter and the differential scanning calorimeter, more advanced chapters on specific applications and tutorials that cover the idiosyncrasies of experimental methods and data analysis. The book draws these together to create the definitive biological calorimetric text book. This book both explains the background to the method and describes novel, high-impact applications. It features works of interest to the experienced calorimetrist and the enthusiastic dilettante. The book should be of interest to all working in the field of biocalorimetry, from graduate students to researchers in academia and in industry.
Author: David Sheehan
Publisher: John Wiley & Sons
Published: 2013-04-30
Total Pages: 433
ISBN-13: 1118687485
DOWNLOAD EBOOK"As will be seen, there is not much missing here. I thought that the sections were well balanced, with rarely too much or too little on a given topic...This is a text to be welcomed by both teachers and students." BIOCHEMISTRY & MOLECULAR BIOLOGY EDUCATION (on the first edition) The second edition of this successful textbook explains the basic principles behind the key techniques currently used in the modern biochemical laboratory and describes the pros and cons of each technique and compares one to another. It is non-mathematical, comprehensive and approachable for students who are not physical chemists. A major update of this comprehensive, accessible introduction to physical biochemistry. Includes two new chapters on proteomics and bioinformatics. Introduces experimental approaches with a minimum of mathematics and numerous practical examples. Provides a bibliography at the end of each chapter. Written by an author with many years teaching and research experience, this text is a must-have for students of biochemistry, biophysics, molecular and life sciences and food science.
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Published: 1990
Total Pages: 812
ISBN-13:
DOWNLOAD EBOOKAuthor: Robert Gustavsson
Publisher: Linköping University Electronic Press
Published: 2018-10-31
Total Pages: 71
ISBN-13: 9176852075
DOWNLOAD EBOOKIn the manufacture of bio-therapeutics the importance of a well-known process is key for a high product titer and low batch to batch variations. Soft sensors are based on the concept that online sensor signals can be used as inputs to mathematical models to derive new valuable process information. This information could then be used for better monitoring and control of the bioprocess. The aim of the present thesis has been to develop soft sensor solutions for upstream bioprocessing and demonstrate their usefulness in improving robustness and increase the batch-to-batch reproducibility in bioprocesses. The thesis reviews the potential and possibilities with soft sensors for use in production of bio-therapeutics to realize FDA´s process analytical technology (PAT) initiative. Modelling and hardware sensor alternatives which could be used in a soft sensor setup are described and critically analyzed. Different soft sensor approaches to control glucose feeding in fed-batch cultures of Escherichia coli are described. Measurements of metabolic fluxes and specific carbon dioxide production was used as control parameters to increase product yield and decrease the variability of produced recombinant proteins. Metabolic heat signals were used in uninduced cultures to estimate and control the specific growth rate at a desired level and thereby also estimate the biomass concentration online. The introduction of sequential filtering of the signal enabled this method to be used in a down-scaled system. The risk and high impact of contaminations in cell cultures are also described. An in situ microscope (ISM) was used as an online tool to estimate cell concentration and also to determine cell diameter size which enabled the detection of contaminant cells at an early stage. The work presented in this thesis supports the idea that soft sensors can be a useful tool in the strive towards robust and reliable bioprocesses, to ensure high product quality and increased economic profit.
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Publisher:
Published: 1992
Total Pages: 1064
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DOWNLOAD EBOOKAuthor: Gennadiĭ Efremovich Zaikov
Publisher: Nova Publishers
Published: 2004
Total Pages: 194
ISBN-13: 9781594541209
DOWNLOAD EBOOKBiotechnology & the Environment Including Biogeotechnology
Author: Christoph A. Schalley
Publisher: John Wiley & Sons
Published: 2007-02-27
Total Pages: 502
ISBN-13: 3527609881
DOWNLOAD EBOOKAn overview of the techniques used to examine supramolecular aggregates from a methodological point of view. Edited by a rising star in the community and an experienced author, this is a definitive survey of useful modern analytical methods for understanding supramolecular chemistry, from NMR to single-molecule spectroscopy, from electron microscopy to extraction methods. A definitive study of this field touching many interdisciplinary areas such as molecular devices, biology, bioorganic chemistry, material science, and nanotechnology.
Author: Marc R. Knecht
Publisher: Springer Science & Business Media
Published: 2014-04-12
Total Pages: 317
ISBN-13: 146149446X
DOWNLOAD EBOOKThis book focuses on the use of bio-inspired and biomimetic methods for the fabrication and activation of nanomaterials. This includes studies concerning the binding of the biomolecules to the surface of inorganic structures, structure/function relationships of the final materials and extensive discussions on the final applications of such biomimetic materials in unique applications including energy harvesting/storage, biomedical diagnostics and materials assembly.
