Basic Cell Culture Protocols

Basic Cell Culture Protocols

Author: Cheryl D. Helgason

Publisher: Humana Press

Published: 2012-11-20

Total Pages: 550

ISBN-13: 9781627031271

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At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems. Written in the highly successful Methods in Molecular BiologyTM, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the essential techniques needed to approach this vital laboratory activity with full success.


Animal Cell Culture Techniques

Animal Cell Culture Techniques

Author: Martin Clynes

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 624

ISBN-13: 3642804128

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Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture.


Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques

Author: Sean R. Gallagher

Publisher: John Wiley & Sons

Published: 2012-03-19

Total Pages: 679

ISBN-13: 047094241X

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The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.


Basic Science Methods for Clinical Researchers

Basic Science Methods for Clinical Researchers

Author: Morteza Jalali

Publisher: Academic Press

Published: 2017-03-31

Total Pages: 383

ISBN-13: 012803078X

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Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow's clinician scientists and future leaders in discovery science. - Serves as a helpful guide for clinical researchers who lack a conventional science background - Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms - Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data - Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)


Cell and Tissue Culture

Cell and Tissue Culture

Author: Alan Doyle

Publisher: Wiley

Published: 1998-11-18

Total Pages: 0

ISBN-13: 9780471982555

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Cell and Tissue Culture: Laboratory Procedures in BiotechnologyEdited by Alan Doyle Centre for Applied Microbiology &Research, Porton Down, Salisbury, UK. and J. Bryan GriffithsScientific Consultancy & Publishing, Porton, Salisbury, UK.Cell and Tissue Culture: Laboratory Procedures in Biotechnologyintroduces the reader to animal cell culture methods describing thekey cells, core techniques, how to scale up the culture forcommercial production, and regulatory aspects. This book provideseasy to follow, step-by-step protocols, with trouble-shooting tipsand notes on time considerations. Alternative procedures,background information and references supplement the mainprocedures described. Other features include: * Experimental examples to indicate expected results; * Quick reference symbols such as safety icons with warning notes;and, * A list of suppliers is provided to allow easy access tolaboratory products. Written by a team of international scientists, Cell and TissueCulture: Laboratory Procedures in Biotechnology will be of interestto researchers, technicians and process engineers using cellculture within the biotechnology, biomedicine and pharmaceuticalindustries.


Animal Cell Culture and Technology

Animal Cell Culture and Technology

Author: Michael Butler

Publisher: Garland Science

Published: 2004-08-02

Total Pages: 299

ISBN-13: 1135325375

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Animal cell culture is an important laboratory technique in the biological and medical sciences. It has become an essential tool for the study of most biochemical and physiological processes and the use of large-scale animal cell culture has become increasingly important to the commercial production of specific compounds for the pharmaceutical industry. This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for undergraduates, graduates and experienced researchers. Reflecting the latest developments and trends in the field, the new topics include the latest theory of the biological clock of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation, and the humanization of antibodies for therapeutic use.


Basic Laboratory Methods for Biotechnology

Basic Laboratory Methods for Biotechnology

Author: Lisa A. Seidman

Publisher: CRC Press

Published: 2021-12-29

Total Pages: 1210

ISBN-13: 1000480763

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Basic Laboratory Methods for Biotechnology, Third Edition is a versatile textbook that provides students with a solid foundation to pursue employment in the biotech industry and can later serve as a practical reference to ensure success at each stage in their career. The authors focus on basic principles and methods while skillfully including recent innovations and industry trends throughout. Fundamental laboratory skills are emphasized, and boxed content provides step by step laboratory method instructions for ease of reference at any point in the students’ progress. Worked through examples and practice problems and solutions assist student comprehension. Coverage includes safety practices and instructions on using common laboratory instruments. Key Features: Provides a valuable reference for laboratory professionals at all stages of their careers. Focuses on basic principles and methods to provide students with the knowledge needed to begin a career in the Biotechnology industry. Describes fundamental laboratory skills. Includes laboratory scenario-based questions that require students to write or discuss their answers to ensure they have mastered the chapter content. Updates reflect recent innovations and regulatory requirements to ensure students stay up to date. Tables, a detailed glossary, practice problems and solutions, case studies and anecdotes provide students with the tools needed to master the content.