Applications of Chimeric Genes and Hybrid Proteins, Part A: Gene Expression and Protein Purification

Applications of Chimeric Genes and Hybrid Proteins, Part A: Gene Expression and Protein Purification

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Publisher: Elsevier

Published: 2000-10-11

Total Pages: 651

ISBN-13: 0080496814

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.


Two-Component Signaling Systems, Part B

Two-Component Signaling Systems, Part B

Author:

Publisher: Academic Press

Published: 2007-07-03

Total Pages: 646

ISBN-13: 0080549462

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Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system." Sections in Two-Component Signaling Systems, Part B include: - Structural Approaches - Reconstitution of Heterogeneous Systems - Intracellular Methods and Assays - Genome-Wide Analyses of Two-Component Systems - Presents detailed protocols - Includes troubleshooting tips


Biothermodynamics Part A

Biothermodynamics Part A

Author: Michael L. Johnson

Publisher: Academic Press

Published: 2009-03-14

Total Pages: 519

ISBN-13: 0080923402

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In the past several years, there has been an explosion in the ability of biologists, molecular biologists and biochemists to collect vast amounts of data on their systems. This volume presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions. The use of thermodynamics in biological research is used as an "energy book-keeping system. While the structure and function of a molecule is important, it is equally important to know what drives the energy force. These methods look to answer: What are the sources of energy that drive the function? Which of the pathways are of biological significance? As the base of macromolecular structures continues to expand through powerful techniques of molecular biology, such as X-ray crystal data and spectroscopy methods, the importance of tested and reliable methods for answering these questions will continue to expand as well.


Biophotonics, Part A

Biophotonics, Part A

Author:

Publisher: Elsevier

Published: 2003-02-19

Total Pages: 751

ISBN-13: 0080522580

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant todaytruly an essential publication for researchers in all fields of life sciences.* Discusses optical instrumentation for imaging, screening and diagnosis in molecules, tissues, and cells* Covers the development and application of optical probes and techniques for imaging and drug screening* Investigates the structure and dynamics of biomolecular systems, screening and drug discovery, and the diagnosis and treatment of disease


Liposomes, Part A

Liposomes, Part A

Author: Nejat Duzgunes

Publisher: Elsevier

Published: 2003-11-11

Total Pages: 345

ISBN-13: 008049708X

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Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies. - Methods in Liposome Preparation - Physiochemical Characterization of Liposomes


Redox Cell Biology and Genetics, Part A

Redox Cell Biology and Genetics, Part A

Author: Chandan K. Sen

Publisher: Elsevier

Published: 2002-07-22

Total Pages: 617

ISBN-13: 0080496997

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant todaytruly an essential publication for researchers in all fields of life sciences.Key Features* Cellular Responses* Tissues and Organs


Chromatin and Chromatin Remodeling Enzymes Part C

Chromatin and Chromatin Remodeling Enzymes Part C

Author: Carl Wu

Publisher: Elsevier

Published: 2004-04-07

Total Pages: 585

ISBN-13: 0080497152

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DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the chromatin remodelling enzymes play an important role in gene transcription. - Genetic assays of chromatin modification and remodeling - Histone modifying enzymes - ATP-dependent chromatin remodeling enzymes


Chromatin and Chromatin Remodeling Enzymes, Part B

Chromatin and Chromatin Remodeling Enzymes, Part B

Author: Carl Wu

Publisher: Elsevier

Published: 2004-03-07

Total Pages: 497

ISBN-13: 0080497144

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DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the Chromatin remodelling enzymes play an important role in gene transcription.


Numerical Computer Methods, Part D

Numerical Computer Methods, Part D

Author:

Publisher: Elsevier

Published: 2004-05-25

Total Pages: 527

ISBN-13: 0080497217

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The aim of Numerical Computer Methods, Part D is to brief researchers of the importance of data analysis in enzymology, and of the modern methods that have developed concomitantly with computer hardware. It is also to validate researchers' computer programs with real and synthetic data to ascertain that the results produced are what they expected. Selected Contents: Prediction of protein structure Modeling and studying proteins with molecular dynamics Statistical error in isothermal titration calorimetry Analysis of circular dichroism data Model comparison methods