Analytical Applications of Immobilized Enzymes with Special Emphasis on Enzyme Electrodes
Author: Hans Nilsson
Publisher:
Published: 1977
Total Pages: 66
ISBN-13:
DOWNLOAD EBOOKRead and Download eBook Full
Author: Hans Nilsson
Publisher:
Published: 1977
Total Pages: 66
ISBN-13:
DOWNLOAD EBOOKAuthor: Lemuel B. Wingard
Publisher: Elsevier
Published: 2014-06-28
Total Pages: 329
ISBN-13: 1483215857
DOWNLOAD EBOOKAnalytical Applications of Immobilized Enzymes and Cells, Volume 3 presents a survey of the interdisciplinary interactions, developments, and trends on research in immobilized enzymes and cells. This volume is comprised of eight chapters. Chapters 1 to 4 are devoted to the description of methodologies and instrumentations which are used in routine analysis. Among those described are enzyme tubes, enzyme thermistors, and enzymic or microbial electrodes. Chapter 4 provides a theoretical analysis of electrode design. Subsequent chapters focus on solid-phase enzyme immunoassays and on techniques for in vivo monitoring of metabolites; economic evaluation of the use of high-purity enzymes in analysis; and an assessment of the significance of microprocessors and computer science in enzyme based analysis. Chemists and chemical engineers will find the book very useful.
Author: George G. Guilbault
Publisher:
Published: 1984
Total Pages: 480
ISBN-13:
DOWNLOAD EBOOKAuthor: George G. Guilbault
Publisher: Springer Science & Business Media
Published: 2012-12-06
Total Pages: 398
ISBN-13: 9400928955
DOWNLOAD EBOOKOn May 4-8, 1987, a NATO Advanced Research Workshop on the Analytical Uses of Immobilized Biological Compounds was held in Florence, Italy. The Director of the Workshop was Professor George G. Guilbault of the University of New Orleans, and the Co-Director was Professor Marco Mascini of the University of Florence It vas the purpose of this meeting to assemble scientists from all NATO Countries with an interest in immobilized biological compounds. to discuss - methods of immobilization - properties of immobilized compounds - enzyme electrodes and biosensors - optical devices utilizing immobilized enzymes - microbial sensors and clinical uses of immobilized enzymes - flow injection analysis using enzymes - immobilized biological compounds in chemical defense detection - pharmaceutical analysis - uses in industrial analysis - enzyme reactors - air pollution detectors - immunosensors - medical uses and applications - solid state and FET sensors Goals to be achieved by the conference were - to permit an exchange of views and experience in all these areas - to review and critically assess the state-of-the-art in these fields - to set guidelines for future research and establish collaborative projects between scientists in NATO laboratories in the above areas. Thirty-seven lectures were given by 36 speakers in all of the above areas.
Author: S. Lam
Publisher: Springer
Published: 1994
Total Pages: 298
ISBN-13:
DOWNLOAD EBOOKEnzymes are bio-catalysts which effect transformation of substrates to products with high specificity. The usage of enzymes in domestic and industrial applications is well known and has been well documented since the early history of civilization. With the advances in understanding of enzymology, usage of enzymes in industrial and biotechnological pro cesses and molecular medicine has proliferated. One of the key factors in the widespread application of enzymes in modern technologies is the development of enzyme immobilization tech niques, which overcome certain practical, functional and economic con straints. Many natural enzymes can be stabilized by immobilization on solid matrices, with most of the activity retained, for a variety of applica tions. An important application of immobilized enzymes is in liquid chromatography. In the last decade, post-column enzyme detection has become established as an important discipline in liquid chromatography. The new detection approach offers more sensitive and specific ways for measuring major classes of biomolecules. Reactors are fabricated by packing the immobilized enzymes into small columns, which can be placed immediately after an HPLC column.
Author: Clarence H. Suelter
Publisher: John Wiley & Sons
Published: 2009-09-25
Total Pages: 279
ISBN-13: 0470110988
DOWNLOAD EBOOKDetails the latest advances in bioanalytical applications using enzymes--techniques that are becoming increasingly important in analysis, synthesis, manufacturing and medical diagnosis. Consists of seven articles which cover: enzyme labeled antibodies in bioassays, DNA restriction enzymes and RFLPs in medicine, enzyme-labeled probes for nucleic acid hybridization, unique methodologies of immobilized proteins in bioanalytical systems, dry reagent chemistry fundamentals, the theory and applications of enzyme electrode biosenors, and advances in enzymatically coupled field effect transistors.
Author: Peter W. Carr
Publisher: John Wiley & Sons
Published: 1980-10-20
Total Pages: 488
ISBN-13:
DOWNLOAD EBOOKAuthor: Thomas Ming Swi Chang
Publisher: Springer Science & Business Media
Published: 2013-11-21
Total Pages: 433
ISBN-13: 1468426109
DOWNLOAD EBOOKI) ADSORPTION EEEEEEEE E E carrier 2) COVALENT LINKAGE a) Insoluble support b) Intermolecular linkage N'E~ ~~ c) Soluble support 0 \:)....m 3) tM TRIX (MOLECULAR) ENTRAPMENT ~~~~~;;..,J~-polymer matrix 4) ENCAPSULATION membrane FIGURE I. Classification of immobilized enzymes. Covalently linked, adsorbed, and matrix-entrapped enzymes represent stage II, research on the microenvironment. Microencapsulation represents stage III, research on the intracellular environment. Further subdivision of microencapsulated enzymes will be found in Chapter 12. 4 T. M. S. CHANG matrix entrapment. In this section, detailed discussions will center on clinical analysis, urine analysis, monitoring of environmental pollution, radioimmune assay, enzyme-linked immunosorbent assay, enzyme electrodes, and other approaches involving immobilized enzymes, antibodies, and antigens. In the final section, research workers describe and discuss the perspectives of immobilized enzymes and proteins. Here, they speculate on the future potential of possible approaches, even though these may not have been extensively studied or tested at the laboratory stage. The biomedical applications of enzymes and proteins, especially in the thera peutic area, is in a very early stage of development. Much remains to be explored and studied, and the area is wide open for investigators interested in original research in a new interdisciplinary area. References Chang, T. M. S., 1972, Artificial Cells, Charles C. Thomas, Publisher, Springfield, Ill. Dunlop, R. B. (ed.), 1974, Immobilized Biochemicals and Affinity ChrOTIULtography, Plenum Press, N ew York.
Author: Henry Freiser
Publisher: Springer Science & Business Media
Published: 2012-12-06
Total Pages: 300
ISBN-13: 1468437763
DOWNLOAD EBOOKWe continue in this second volume the plan evident in the first; i.e., of presenting a number of well-rounded up-to-date reviews of important developments in the exciting field of ion-selective electrodes in analytical chemistry. In this volume, in addition to the exciting applications of ISE'S to biochemistry systems represented by the description of enzyme electrodes, there is featured the most recent development in ISE'S, namely, the joining of the electrochemical and solid state expertise, resulting in CHEMFETS. The scholarly survey of the current status of ISE'S will undoubtedly be welcomed by all workers in the field. Tucson, Arizona Henry Freiser vii Contents Chapter 1 Potentiometric Enzyme Methods Robert K. Kobos 1. Introduction . . . . . . 1 2. Soluble Enzyme Systems . . . 5 2.1. Substrate Determinations 5 2.2. Enzyme Determinations . 13 2.3. Inhibitor Determinations. 18 3. Immobilized Enzyme Systems . 19 3.1. Methods of Immobilization. 19 3.2. Characteristics of Immobilized Enzymes 23 3.3. Analytical Applications with Ion-Selective Electrodes 23 4. Enzyme Electrodes 31 4.1. Urea Electrodes 35 4.2. Amygdalin Electrodes 39 4.3. Glucose Electrodes . 40 4.4. Penicillin Electrodes 40 4.5. Amino Acid Electrodes 41 4.6. Nucleotide Electrodes 46 4.7. Uric Acid Electrode 47 4.8. Creatinine Electrode 48 48 4.9. Acetylcholine Electrodes. 4.10. D-Gluconate Electrode 49 4.11. Lactate Electrode 49 4.12. Inhibitor Determination 50 4.13. Substrate Electrodes 50 4.14. Current Trends . . . .
Author: José M. Guisán
Publisher: Springer Science & Business Media
Published: 2008-02-05
Total Pages: 447
ISBN-13: 1597450537
DOWNLOAD EBOOKEnzymes and whole cells are able to catalyze the most complex chemical processes under the most benign experimental and environmental conditions. In this way, enzymes and cells could be excellent catalysts for a much more sustainable chemical industry. However, enzymes and cells also have some limitations for nonbiological applications: fine chemistry, food chemistry, analysis, therapeutics, and so on. Enzymes and cells may be unstable, difficult to handle under nonconventional conditions, poorly selective toward synthetic substrates, and so forth. From this point of view, the transformation—from the laboratory to industry—of chemical processes catalyzed by enzymes and cells may be one of the most complex and exciting goals in biotechnology. For many industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used over very long periods of time. From this point of view, immobilization, simplicity, and stabilization have to be strongly related concepts. Over the last 30 years, a number of protocols for the immobilization of cells and enzymes have been reported in scientific literature. However, only very few protocols are simple and useful enough to greatly improve the functional properties of enzymes and cells, activity, stability, selectivity, and related properties.