Diversity in Barley (Hordeum vulgare)

Diversity in Barley (Hordeum vulgare)

Author: R. von Bothmer

Publisher: Elsevier

Published: 2003-07-03

Total Pages: 307

ISBN-13: 0080530478

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Genetic diversity is one of the main resources sustaining human life. Food security largely depends on the availability and utilization of this diversity, which is of strategic importance for countries and companies. Conservation and utilization of biodiversity is thus currently an urgent area of global debate and concern. Barley is a major crop in the world used for food, feed and malt, and with a wide religious and ethnic importance. The crop was domesticated in Neolithic time in SW Asia and spread rapidly under cultivation to new areas. Nowadays it is one of the most widespread and widely adapted crops grown under contrasting edaphic conditions. Adaptations to new environments, different agricultural practices and selection for different uses have further added to the complex diversity pattern. Is it at all possible to give a complete picture of the diversity in a crop or wild species? Are we, by adding new technologies, only revealing parts of the diversity? Do different sets of data show similar or conflicting pictures of genetic diversity? Will the large genome size reduce the role of barley as a model organism in these current sequencing days? Or, are there still major reasons to continue to work with this beautiful crop? The aim of this book is to cover the complex issue of diversification in time and space in a single crop: barley. Leading scientists from various fields describe the entire variation pattern in different sets of characters and an attempt is made for a synthesis to a holistic picture. The book proposes ways to use the achievements of diversity studies in future research and breeding programmes.


The Barley Genome

The Barley Genome

Author: Nils Stein

Publisher: Springer

Published: 2018-08-18

Total Pages: 400

ISBN-13: 3319925288

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This book presents an overview of the state-of-the-art in barley genome analysis, covering all aspects of sequencing the genome and translating this important information into new knowledge in basic and applied crop plant biology and new tools for research and crop improvement. Unlimited access to a high-quality reference sequence is removing one of the major constraints in basic and applied research. This book summarizes the advanced knowledge of the composition of the barley genome, its genes and the much larger non-coding part of the genome, and how this information facilitates studying the specific characteristics of barley. One of the oldest domesticated crops, barley is the small grain cereal species that is best adapted to the highest altitudes and latitudes, and it exhibits the greatest tolerance to most abiotic stresses. With comprehensive access to the genome sequence, barley’s importance as a genetic model in comparative studies on crop species like wheat, rye, oats and even rice is likely to increase.


Molecular Tools for Screening Biodiversity

Molecular Tools for Screening Biodiversity

Author: A. Karp

Publisher: Springer Science & Business Media

Published: 2012-12-06

Total Pages: 482

ISBN-13: 9400900198

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Mark Chase There are many literature resources available to molecular biologists wishing to assess genetic variation, but the myriad of techniques and approaches potentially available to the plant breeder and the evolutionary biologist is truly bewildering, and most have never been evaluated side-by-side on the same sets of samples. Additionally, it is often not recognized that tools that are useful for breeders can often be adapted for use in evolutionary studies and vice versa, but this is generally the case. The borderline between population genetics and phylogenetics is vague and difficult to assess, and a combination of both types of tools is best when it is not clear with which area one is dealing. Furthermore, it is not now appropriate to use just one type of marker in any kind of study; most markers have the potential to misinform under certain conditions, so it is always wise to incorporate at least two different types of assessments into any project. This volume is designed to facilitate this sort of multiple approach and provides comparative data on most currently available methods so that researchers can more intelligently select those appropriate to their area of interest, regardless of whether it is in the realm of breeding or evolutionary biology.


Exploiting Hordeum Vulgare Subsp. Spontaneum Genetic Resources

Exploiting Hordeum Vulgare Subsp. Spontaneum Genetic Resources

Author: Iván Ariel Matus

Publisher:

Published: 2002

Total Pages: 228

ISBN-13:

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Wild barley (Hordeum vulgare subsp. spontaneum) could be a source of useful genes for improving cultivated barley. The useful genes present in Hordeum vulgare subsp. spontaneum may be new alleles at described loci, or these may be entirely new genes in the sense that there is limited allelic variation at these loci in the cultivated germplasm pool. This research was directed at gene discovery in wild barley and involved two steps: (i) characterization of diversity using genetic markers and (ii) development and characterization of novel germplasm for gene discovery. Simple Sequence Repeats (SSRs) of known map location were used to survey three representative groups of barley germplasm: a sample of crop progenitor (Hordeum vulgare subsp. spontaneum) accessions, a group of mapping population parents, and a group of varieties and elite breeding lines. The objectives were to determine the informativeness and utility of SSRs in differentiating and classifying the three sets of barley germplasm. Crop progenitors had the highest number of alleles per SSR locus, followed by mapping population parents and elite breeding lines. The cluster analysis indicated a high level of diversity within the crop progenitor accessions and within the mapping population parents. It revealed a much lower level of diversity within the elite breeding germplasm. A set of Recombinant Chromosome Substitution Lines (RCSLs) representing introgressions of Hordeum vulgare subsp. spontaneum genome in to a cultivated barley background were developed using the Advanced Backcross strategy. An accession of Hordeum vulgare subsp. spontaneum was the donor parent and the variety "Harrington" was the recurrent parent. The RCSLs were developed via two backcrosses to the recurrent parent followed by six generations of selfing. The genomic architecture of the RCSLs was determined by molecular marker fingerprinting with SSRs. The consequences of introgressions of Hordeum vulgare subsp. spontaneum genome segments into the recurrent parent were assessed in terms of inflorescence yield components, malting quality traits, and domestication-related traits. Hordeum spontaneum subsp. spontaneum, despite its overall inferior phenotype, contributed favorable alleles for some characters of agronomic performance. In other cases, the introgressions caused a disruption of the Harrington phenotype, a "reverse genetics" approach to gene discovery.