Advanced Techniques in Plant Sciences

Advanced Techniques in Plant Sciences

Author: Dr. D. A. Dhale

Publisher: Book Saga Publications

Published: 2023-05-28

Total Pages: 316

ISBN-13: 9395470429

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This book, ‘Advanced Techniques in Plant Sciences’ includes basic and advanced techniques like microscopy, polarizing microscopy, transmission and scanning electron microscopes, confocal microscopy, phase contrast microscopy, photomicrography, digital imaging, and microscopic measurements. The Spectroscopic techniques contain Colorimeter, Spectroscopic techniques, Ultraviolet-visible spectroscopy; Infrared spectroscopy, Nuclear magnetic resonance spectroscopy; Basic NMR techniques, NMR Imaging Principles, Electron Spin Resonance (ESR) Spectroscopy, Atomic Absorption Spectroscopy (AAS); Mass spectrometry, Flame photometer, etc. In histochemical studies, various methods include proteins, carbohydrates, lipids, and enzymes; how to prepare the macrolides; and the different methods of preparing the slides, which include Along with this, you will also study whole mounts. Also, about the sectioning of plant specimens, free hand, microtome, rotary microtome sectioning, types of microtome, and methods of serial sectioning. Maceration, Gel electrophoresis, PAGE, SDS-PAGE, Agarose gel electrophoresis; isoelectric focusing, and 2D electrophoresis Blotting Techniques: Principles and Techniques of Southern, Northern, and Western Blotting Techniques and Southwestern Blotting. Centrifugation techniques, Types of Centrifugation, Ultracentrifugation, Concept of Partition Coefficient; Chromatography, Techniques by chromatographic bed shape, Paper Chromatography, Double-way paper chromatography, Planar chromatography, Thin layer chromatography (TLC), High-performance liquid chromatography (HPLC)- Partition chromatography, Normal-phase chromatography, Displacement chromatography, Reversed-phase chromatography (RPC), Size-exclusion chromatography, Ion-exchange chromatography, Bioaffinity chromatography, Aqueous normal-phase chromatography, Isocratic flow, and gradient elution; High-Performance Thin-Layer Chromatography (HPTLC), Gas chromatography (GC), Flash column chromatography, Column chromatography, Ion chromatography, Gel-filtration chromatography or Size-exclusion chromatography, Pyrolysis gas chromatography, Countercurrent chromatography.


Technological Advancements in Plant Sciences

Technological Advancements in Plant Sciences

Author: Raghvendra Pratap Narayan

Publisher: Nova Science Publishers

Published: 2016

Total Pages: 0

ISBN-13: 9781536100044

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Botany is a forerunning field of study that could address the foremost disputes facing humanity in the 21st century; coupled with the development of new tools and techniques, it could aid in finding solutions to diagnose these unsolved questions. The present book summarises the recent technological advances of plant science. This is a succinct, up-to-date, and relevant compilation based on the techniques used in plant science; this book embodies the recent knowledge of advanced techniques frequently being used from last few decades. There are twelve chapters summarised systematically that demonstrate: the suitability of Laser Produced Plasma Spectroscopy; potential of NMR spectroscopy in plant metabolomics; recent developments and applications of novel analytical techniques for the analysis of plant materials; chlorophyll as a fluorescence analysis and a technique to explore the photochemistry of photosystem II; drought stress and metabolomics in plants; heat shock on Bipolaris sorokiniana in cereal crops; salt stress on crops plants and their mitigation strategies; plant cell death by rumen-induced stress on plant in ruminant herbivores; the role of diazotrophs in maintaining plant health in agricultural fields; population biology of Rhizoctonia solani AG-1 IA from India; and biofilms as a home for microorganisms and genetically modified organism. This book will be helpful for researchers, academicians and students working on the related fields.


Genome Engineering for Crop Improvement

Genome Engineering for Crop Improvement

Author: Bidyut Kumar Sarmah

Publisher: Springer Nature

Published: 2021-04-18

Total Pages: 277

ISBN-13: 3030633721

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This book serves the teachers, researchers and the students as a handy and concise reference as well as guidebook while designing and planning for use of the advanced technologies for crop improvement. The content of the book is designed to cover the latest genome engineering techniques for crop improvement. The conventional breeding has got its limitations such as non-availability of desired genes within the genepool. In many cases, breeding has been highly used and it has nearly reached its highest limit so far as the productivity and production of crops are concerned. However, with increasing need of food and decreasing resources, including water, land, labour, etc., to feed the growing population, the alternative available ways of increasing crop productivity need to be explored and exploited. Genome engineering has a wide scope that includes technologies such as genetic engineering and transgenesis, RNA technologies, CRISPR, cisgenics and subgenics for better productivity and more efficient biotic and abiotic stress management. Therefore, the book is planned to enlighten the readers with the advanced technologies with examples and case studies, whenever possible. Efforts will be made to emphasize on general efforts on various major food crops; however, it would also be made clear that such efforts could be taken as proofs of concepts and that this could be extrapolated keeping the demand in mind.


New Directions for Biosciences Research in Agriculture

New Directions for Biosciences Research in Agriculture

Author: National Research Council

Publisher: National Academies Press

Published: 1985-01-01

Total Pages: 136

ISBN-13: 0309035422

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Authored by an integrated committee of plant and animal scientists, this review of newer molecular genetic techniques and traditional research methods is presented as a compilation of high-reward opportunities for agricultural research. Directed to the Agricultural Research Service and the agricultural research community at large, the volume discusses biosciences research in genetic engineering, animal science, plant science, and plant diseases and insect pests. An optimal climate for productive research is discussed.


Biosensors in Agriculture: Recent Trends and Future Perspectives

Biosensors in Agriculture: Recent Trends and Future Perspectives

Author: Ramesh Namdeo Pudake

Publisher: Springer Nature

Published: 2021-03-12

Total Pages: 496

ISBN-13: 3030661652

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This book reviews the application of nanosensors in food and agriculture. Nanotechnology has the potential to become transformative technology that will impact almost all sectors. Tools like nanosensors, which detect specific molecular interactions, can be used for on-site, in-situ and online measurements of various parameters in clinical diagnostics, environmental and food monitoring, and quality control. Due to their unprecedented performance and sensitivity, nanobiosensors are gaining importance in precision farming. The book examines the use of nanobiosensors in the monitoring of food additives, toxins and mycotoxins, microbial contamination, food allergens, nutritional constituents, pesticides, environmental parameters, plant diseases and genetically modified organisms. It also discusses the role of biosensors in increasing crop productivity in sustainable agriculture, and nanosensor-based smart delivery systems to optimize the use of natural resources such as water, nutrients and agrochemicals in precision farming.


Modern Applications of Plant Biotechnology in Pharmaceutical Sciences

Modern Applications of Plant Biotechnology in Pharmaceutical Sciences

Author: Saurabh Bhatia

Publisher: Academic Press

Published: 2015-07-22

Total Pages: 452

ISBN-13: 0128024984

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Modern Applications of Plant Biotechnology in Pharmaceutical Sciences explores advanced techniques in plant biotechnology, their applications to pharmaceutical sciences, and how these methods can lead to more effective, safe, and affordable drugs. The book covers modern approaches in a practical, step-by-step manner, and includes illustrations, examples, and case studies to enhance understanding. Key topics include plant-made pharmaceuticals, classical and non-classical techniques for secondary metabolite production in plant cell culture and their relevance to pharmaceutical science, edible vaccines, novel delivery systems for plant-based products, international industry regulatory guidelines, and more. Readers will find the book to be a comprehensive and valuable resource for the study of modern plant biotechnology approaches and their pharmaceutical applications. - Builds upon the basic concepts of cell and plant tissue culture and recombinant DNA technology to better illustrate the modern and potential applications of plant biotechnology to the pharmaceutical sciences - Provides detailed yet practical coverage of complex techniques, such as micropropogation, gene transfer, and biosynthesis - Examines critical issues of international importance and offers real-life examples and potential solutions


Intelligent Image Analysis for Plant Phenotyping

Intelligent Image Analysis for Plant Phenotyping

Author: Ashok Samal

Publisher: CRC Press

Published: 2020-10-21

Total Pages: 347

ISBN-13: 1351709992

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Domesticated crops are the result of artificial selection for particular phenotypes or, in some cases, natural selection for an adaptive trait. Plant traits can be identified through image-based plant phenotyping, a process that was, until recently, strenous and time-consuming. Intelligent Image Analysis for Plant Phenotyping reviews information on time-saving techniques, using computer vision and imaging technologies. These methodologies provide an automated, non-invasive, and scalable mechanism by which to define and collect plant phenotypes. Beautifully illustrated, with numerous color images, the book focuses on phenotypes measured from individual plants under controlled experimental conditions, which are widely available in high-throughput systems. Features: Presents methodologies for image processing, including data-driven and machine learning techniques for plant phenotyping. Features information on advanced techniques for extracting phenotypes through images and image sequences captured in a variety of modalities. Includes real-world scientific problems, including predicting yield by modeling interactions between plant data and environmental information. Discusses the challenge of translating images into biologically informative quantitative phenotypes. A practical resource for students, researchers, and practitioners, this book is invaluable for those working in the emerging fields at the intersection of computer vision and plant sciences.


Safety of Genetically Engineered Foods

Safety of Genetically Engineered Foods

Author: National Research Council

Publisher: National Academies Press

Published: 2004-07-08

Total Pages: 254

ISBN-13: 0309166152

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Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.


Genetic Engineering of Plants

Genetic Engineering of Plants

Author: National Research Council

Publisher: National Academies Press

Published: 1984-02-01

Total Pages: 97

ISBN-13: 0309034345

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"The book...is, in fact, a short text on the many practical problems...associated with translating the explosion in basic biotechnological research into the next Green Revolution," explains Economic Botany. The book is "a concise and accurate narrative, that also manages to be interesting and personal...a splendid little book." Biotechnology states, "Because of the clarity with which it is written, this thin volume makes a major contribution to improving public understanding of genetic engineering's potential for enlarging the world's food supply...and can be profitably read by practically anyone interested in application of molecular biology to improvement of productivity in agriculture."


Recent Advances in Plant Science

Recent Advances in Plant Science

Author: Yıldız Aydın

Publisher:

Published: 2020

Total Pages: 369

ISBN-13: 9781536172652

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This book compiles original and review advances from a number of different focuses and latest developments in the important field of plant biology/science from around the world. The publication will be a beneficial and valuable resource for many people and groups related to plant growth and development as well as teachers, researchers, commercial growers and advanced students of plant biological science. The proposed publication can be used in some interesting and unusual places such as biofuels, edible vaccine, phytoremediation and cosmetics.